Fluorinated PEG-Polypeptide Polyplex Micelles Have Good Serum-Resistance and Low Cytotoxicity for Gene Delivery

Macromol Biosci. 2017 Aug;17(8). doi: 10.1002/mabi.201700114. Epub 2017 May 19.

Abstract

A novel PEGylation polypeptide, poly(ethylene glycol)-b-poly(l-lysine)-b-poly(l-cysteine) (PEG-PLL-PCys) triblock copolymer is synthesized via the sequential ring-opening polymerization of amino acid N-carboxyanhydrides initiated by methoxypolyethylene glycol amine (mPEG-NH2 , Mw is 2 kDa). Subsequently, the obtained polypeptide is partially conjugated with fluorocarbon chains via disulfide exchange reaction. PLL segment can condense plasmid DNA through an electrostatic force to form a complex core, PEG segment surrounding the complex like a corona can prevent the complex from precipitation and reduce the adsorption of serum, while PCys segment with fluorocarbon can enhance the cellular uptake and the stability of the formed polyplex micelles in physiological conditions. Experiment results exhibit that the fluorinated polypeptides have low cytotoxicity and good gene transfection efficiency even in the presence of 50% fetal bovine serum.

Keywords: fluorocarbon chains; gene delivery; polypeptides; polyplex micelles; serum-resistance.

MeSH terms

  • Gene Transfer Techniques*
  • HeLa Cells
  • Humans
  • Materials Testing
  • Micelles*
  • Peptides* / chemistry
  • Peptides* / pharmacology
  • Plasmids* / chemistry
  • Plasmids* / pharmacology
  • Polyethylene Glycols* / chemistry
  • Polyethylene Glycols* / pharmacology
  • Protein Corona / chemistry*

Substances

  • Micelles
  • Peptides
  • Protein Corona
  • Polyethylene Glycols