In this study, polyanionic alginate gel beads crosslinked by Ca2+ and glutaraldehyde have demonstrated a strong electrostatic interaction with specific proteins. Due to the naturally abundant carboxyl groups, the prepared alginate gel beads exhibited a relatively superior integrated adsorption performance toward lysozyme, including a superior adsorption capacity of 213mgg-1, fast adsorption equilibrium within 12h, good selectivity, and good reversibility. Compared with other protein adsorbents, the as-prepared adsorptive beads have the advantages of excellent adsorption performance, easy to prepare, convenient, efficient, reliable and environment-friendly to apply, which can serve as a more sustainable material in protein separation and purification.
Keywords: Adsorption; Alginate; Calcium; Electrostatic interaction; Lysozyme.
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