Genetic characterization of a Neisseria meningitidis cluster in Queensland, Australia

Can J Microbiol. 2017 Jul;63(7):644-647. doi: 10.1139/cjm-2017-0017. Epub 2017 May 1.

Abstract

Neisseria meningitidis serogroups B and C have been responsible for the majority of invasive meningococcal disease in Australia, with serogroup B strains causing an increasing proportion of cases in recent years. Serogroup Y has typically caused sporadic disease in Australia. In 2002, a cluster of 4 cases was reported from a rural region in Queensland. Three of these cases were serogroup C, with 1 case diagnosed by molecular detection only, and the fourth case was identified as a serogroup Y infection. Genomic analysis, including antigen finetyping, multilocus sequence typing (MLST), and core genome MLST, demonstrated that the serogroup Y case, though spatially and temporally linked to a serogroup C disease cluster, was not the product of a capsule switch and that one of the serogroup C isolates had a deletion of the entire porA sequence.

Keywords: Neisseria meningitidis; mutant porA; outbreak; porA mutant; séquençage du génome entier; whole genome sequencing; éclosion.

MeSH terms

  • Cluster Analysis
  • Disease Outbreaks*
  • Humans
  • Meningococcal Infections / epidemiology
  • Meningococcal Infections / microbiology*
  • Multilocus Sequence Typing
  • Neisseria meningitidis / genetics*
  • Neisseria meningitidis / immunology
  • Neisseria meningitidis / isolation & purification
  • Porins / genetics*
  • Queensland
  • Sequence Analysis, DNA
  • Serogroup

Substances

  • Porins
  • porin protein, Neisseria