Tetraphenylethene derivative modified DNA oligonucleotide for in situ potassium ion detection and imaging in living cells

Abstract

The monitoring of K⁺ is very important and emergency because of their unique relationship in various disease diagnosis and treatment. G-quadruplex analogue is a classical recognition unit for K⁺ detection and has been widely applied in K⁺ relevant research. Common fluorescent dyes were employed for design of G-quadruplex structure-based K⁺ probes which suffered from the aggregation-caused quenching effect, and possibly limited the biological applications in living systems. Herein, we report an aggregation-induced emission (AIE) effect-based fluorescent probe for cellular K⁺ analysis and imaging. Benefitting from the K⁺ triggered AIE phenomenon, the designed TPE derivative modified guanine (G)-rich oligonucleotide fluorescent probe (TPE-oligonucleotide probe) exhibits high sensitivity (∼10-fold higher than most reported G-quadruplex-based probes) with extended photostability which facilitates the prolonged fluorescence observations of K⁺ in living cells. On the basis of these advantages, the TPE-oligonucleotide probe serves as a promising candidate for the functional study and analysis of K⁺.

Keywords: Aggregation-induced emission; Cell imaging; G-quadruplex analogue; Potassium ion detection; Tetraphenylethene derivative; Turn-on response.