Transcriptomic analyses and leukocyte telomere length measurement in subjects exposed to severe recent stressful life events

Transl Psychiatry. 2017 Feb 21;7(2):e1042. doi: 10.1038/tp.2017.5.

Abstract

Stressful life events occurring in adulthood have been found able to affect mood and behavior, thus increasing the vulnerability for several stress-related psychiatric disorders. However, although there is plenty of clinical data supporting an association between stressful life events in adulthood and an enhanced vulnerability for psychopathology, the underlying molecular mechanisms are still poorly investigated. Thus, in this study we performed peripheral/whole-genome transcriptomic analyses in blood samples obtained from 53 adult subjects characterized for recent stressful life events occurred within the previous 6 months. Transcriptomic data were analyzed using Partek Genomics Suite; pathway and network analyses were performed using Ingenuity Pathway Analysis and GeneMANIA Software. We found 207 genes significantly differentially expressed in adult subjects who reported recent stressful life experiences (n=21) compared with those without such experiences (n=32). Moreover, the same subjects exposed to such stressful experiences showed a reduction in leukocyte telomere length. A correlation analyses between telomere length and transcriptomic data indicated an association between the exposures to recent stressful life events and the modulation of several pathways, mainly involved in immune-inflammatory-related processes and oxidative stress, such as natural killer cell signaling, interleukin-1 (IL-1) signaling, MIF regulation of innate immunity and IL-6 signaling. Our data suggest an association between exposures to recent stressful life events in adulthood and alterations in the immune, inflammatory and oxidative stress pathways, which could be also involved in the negative effect of stressful life events on leukocyte telomere length. The modulation of these mechanisms may underlie the clinical association between the exposure to recent Stressful life events in adulthood and an enhanced vulnerability to develop psychiatric diseases in adulthood.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Case-Control Studies
  • Female
  • Gene Expression Profiling
  • Gene Expression Regulation
  • Humans
  • Hydrocortisone / metabolism
  • Immunity, Innate / genetics
  • Inflammation / genetics*
  • Interleukin-1 / genetics
  • Interleukin-6 / metabolism
  • Intramolecular Oxidoreductases / metabolism
  • Leukocytes / metabolism*
  • Life Change Events*
  • Linear Models
  • Macrophage Migration-Inhibitory Factors / metabolism
  • Male
  • Oxidative Stress / genetics*
  • Real-Time Polymerase Chain Reaction
  • Signal Transduction / genetics
  • Telomere / metabolism*

Substances

  • IL6 protein, human
  • Interleukin-1
  • Interleukin-6
  • Macrophage Migration-Inhibitory Factors
  • Intramolecular Oxidoreductases
  • MIF protein, human
  • Hydrocortisone