Assessment of Corneal Sensation, Innervation and Retinal Nerve Fiber Layer in Patients Treated with Multiple Intravitreal Ranibizumab Injections

PLoS One. 2017 Jan 13;12(1):e0170271. doi: 10.1371/journal.pone.0170271. eCollection 2017.

Abstract

Purpose: To evaluate the effects of repeated intravitreal ranibizumab injections on corneal sensitivity, corneal sub-basal nerve plexus (SBNP) and peripapillary retinal nerve fiber layer (RNFL) thickness in patients with neovascular age-related macular degeneration (AMD).

Methods: Sixty-six eyes of 33 patients who had received unilateral repeated intravitreal ranibizumab injections (0.5 mg/0.05 ml) for the treatment of AMD and 25 eyes of 25 healthy subjects were included in the study. Central corneal sensation was measured using the contact Cochet-Bonnet esthesiometer. The laser scanning in vivo corneal confocal microscope was used to determine corneal SBNP parameters. The peripapillary RNFL thickness was assessed with spectral-domain optical coherence tomography. Data obtained from the ranibizumab-injected eyes were compared with those of the fellow non-treated eyes and the eyes of the healthy control subjects.

Results: The mean number of ranibizumab injections per eye was 8.9±5.0 (range 3-20). There were no statistically significant differences in the central corneal sensitivity threshold and corneal SBNP parameters between the ranibizumab-injected eyes and the fellow untreated eyes or between those with neovascular AMD and the healthy control group (P>0.05 for all). The average peripapillary RNFL thickness of the treated eyes did not differ significantly to the fellow eyes (P = 0.237), and the eyes of healthy control subjects (P = 0.918). There were no significant correlations between the number of ranibizumab injections and any of the study parameters.

Conclusions: Multiple intravitreal injections of ranibizumab seem to have no harmful effects on corneal sensitivity, innervation and peripapillary RNFL thickness in patients with AMD.

MeSH terms

  • Aged
  • Aged, 80 and over
  • Cornea / drug effects*
  • Cornea / innervation
  • Cornea / physiopathology
  • Female
  • Humans
  • Intravitreal Injections
  • Macular Degeneration / drug therapy*
  • Macular Degeneration / pathology
  • Male
  • Microscopy, Confocal
  • Middle Aged
  • Nerve Fibers / drug effects
  • Nerve Fibers / ultrastructure
  • Ranibizumab / administration & dosage
  • Ranibizumab / adverse effects
  • Ranibizumab / therapeutic use*
  • Retina / drug effects*
  • Retina / pathology
  • Retina / ultrastructure

Substances

  • Ranibizumab

Grants and funding

This work was supported by the Scientific Research Fund of the Necmettin Erbakan University (Project Number: 151218003) (GB). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.