Overexpression of PTP4A3 in ETV6-RUNX1 acute lymphoblastic leukemia

Toni Grönroos; Susanna Teppo; Juha Mehtonen; Saara Laukkanen; Thomas Liuksiala; Matti Nykter; Merja Heinäniemi; Olli Lohi

doi:10.1016/j.leukres.2016.12.005

Overexpression of PTP4A3 in ETV6-RUNX1 acute lymphoblastic leukemia

Leuk Res. 2017 Mar:54:1-6. doi: 10.1016/j.leukres.2016.12.005. Epub 2016 Dec 26.

Authors

Toni Grönroos¹, Susanna Teppo², Juha Mehtonen³, Saara Laukkanen², Thomas Liuksiala⁴, Matti Nykter⁵, Merja Heinäniemi³, Olli Lohi²

Affiliations

¹ Tampere Center for Child Health Research, University of Tampere and Tampere University Hospital, Tampere, Finland. Electronic address: toni.gronroos@staff.uta.fi.
² Tampere Center for Child Health Research, University of Tampere and Tampere University Hospital, Tampere, Finland.
³ Institute of Biomedicine, School of Medicine, University of Eastern Finland, Kuopio, Finland.
⁴ Tampere Center for Child Health Research, University of Tampere and Tampere University Hospital, Tampere, Finland; Institute of Biosciences and Medical Technology, University of Tampere, Finland.
⁵ Institute of Biosciences and Medical Technology, University of Tampere, Finland; Department of Signal Processing, Tampere University of Technology, Tampere, Finland.

PMID: 28063378
DOI: 10.1016/j.leukres.2016.12.005

Abstract

Cell signalling, which is often derailed in cancer, is a network of multiple interconnected pathways with numerous feedback mechanisms. Dynamics of cell signalling is intimately regulated by addition and removal of phosphate groups by kinases and phosphatases. We examined expression of members of the PTP4A family of phosphatases across acute leukemias. While expression of PTP4A1 and PTP4A2 remained relatively unchanged across diseases, PTP4A3 showed marked overexpression in ETV6-RUNX1 and BCR-ABL1 subtypes of precursor B cell acute lymphoblastic leukemia. We show that PTP4A3 is regulated by the ETV6-RUNX1 fusion, but noticed no marked impact on cell viability either after PTP4A3 silencing or treatment with a PTP4A3 inhibitor. Regulation of PTP4A3 expression is altered in specific subgroups of acute leukemias and this is likely brought about by expression of the aberrant fusion genes.

Keywords: ETV6-RUNX1; Leukemia; PTP4A3.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line
Cell Survival
Core Binding Factor Alpha 2 Subunit*
Fusion Proteins, bcr-abl
Gene Expression Regulation, Neoplastic*
Humans
Neoplasm Proteins / genetics*
Oncogene Proteins, Fusion*
Precursor Cell Lymphoblastic Leukemia-Lymphoma / genetics*
Protein Tyrosine Phosphatases / genetics*

Substances

Core Binding Factor Alpha 2 Subunit
Neoplasm Proteins
Oncogene Proteins, Fusion
TEL-AML1 fusion protein
Fusion Proteins, bcr-abl
PTP4A3 protein, human
Protein Tyrosine Phosphatases