Identification and functional characterization of a novel MTFMT mutation associated with selective vulnerability of the visual pathway and a mild neurological phenotype

Neurogenetics. 2017 Apr;18(2):97-103. doi: 10.1007/s10048-016-0506-0. Epub 2017 Jan 5.

Abstract

Mitochondrial protein synthesis is initiated by formylated tRNA-methionine, which requires the activity of MTFMT, a methionyl-tRNA formyltransferase. Mutations in MTFMT have been associated with Leigh syndrome, early-onset mitochondrial leukoencephalopathy, microcephaly, ataxia, and cardiomyopathy. We identified compound heterozygous MTFMT mutations in a patient with a mild neurological phenotype and late-onset progressive visual impairment. MRI studies documented a progressive and selective involvement of the retrochiasmatic visual pathway. MTFMT was undetectable by immunoblot analysis of patient fibroblasts, resulting in specific defects in mitochondrial protein synthesis and assembly of the oxidative phosphorylation complexes. This report expands the clinical and MRI phenotypes associated with MTFMT mutations, illustrating the complexity of genotype-phenotype relationships in mitochondrial translation disorders.

Keywords: Leukoencephalopathy; MTFMT; OXPHOS defects; Optic pathway; Whole exome sequencing.

Publication types

  • Case Reports

MeSH terms

  • Cognitive Dysfunction / complications
  • Cognitive Dysfunction / genetics*
  • DNA Mutational Analysis
  • Female
  • Humans
  • Hydroxymethyl and Formyl Transferases / genetics*
  • Mitochondrial Diseases / complications
  • Mitochondrial Diseases / genetics*
  • Phenotype
  • Vision Disorders / genetics*
  • Visual Pathways / metabolism
  • Visual Pathways / pathology
  • Young Adult

Substances

  • Hydroxymethyl and Formyl Transferases
  • methionyl-tRNA formyltransferase