Identification of a cyclodextrin inclusion complex of antimicrobial peptide CM4 and its antimicrobial activity

Jian-Feng Li; Jia-Xin Zhang; Zhi-Guo Wang; Ying-Jia Yao; Xu Han; You-Long Zhao; Jun-Ping Liu; Shuang-Quan Zhang

doi:10.1016/j.foodchem.2016.10.040

Identification of a cyclodextrin inclusion complex of antimicrobial peptide CM4 and its antimicrobial activity

Food Chem. 2017 Apr 15:221:296-301. doi: 10.1016/j.foodchem.2016.10.040. Epub 2016 Oct 11.

Authors

Jian-Feng Li¹, Jia-Xin Zhang², Zhi-Guo Wang³, Ying-Jia Yao⁴, Xu Han⁵, You-Long Zhao⁶, Jun-Ping Liu³, Shuang-Quan Zhang⁶

Affiliations

¹ Institute of Aging Research, School of Medicine, Hangzhou Normal University, Hangzhou, Zhejiang Province 311121, China; Jiangsu Province Key Laboratory for Molecular and Medical Biotechnology, Life Sciences College, Nanjing Normal University, Nanjing, Jiangsu Province 210046, China. Electronic address: lijianfeng@hznu.edu.cn.
² Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing, Jiangsu Province 210037, China.
³ Institute of Aging Research, School of Medicine, Hangzhou Normal University, Hangzhou, Zhejiang Province 311121, China.
⁴ Nursing, Lishui University (Sino-forsign cooperation), Lishui, Zhejiang Province 323499, China.
⁵ Guangdong University of Education, Guangzhou, Guangdong Province 510303, China.
⁶ Jiangsu Province Key Laboratory for Molecular and Medical Biotechnology, Life Sciences College, Nanjing Normal University, Nanjing, Jiangsu Province 210046, China.

PMID: 27979206
DOI: 10.1016/j.foodchem.2016.10.040

Abstract

Antibacterial peptide CM4 (ABP-CM4) is a natural product isolated from the silkworm Bombyx mori. It is a small cationic peptide with broad-spectrum activities against harmful microorganisms and may be used as a novel food preservative. However, ABP-CM4 lacks tertiary structure in water-like solutions, which makes it more susceptible to proteases and labile when exposed to air. In this study, β-cyclodextrin (β-CD) was chosen to form an inclusion complex with ABP-CM4, which enhanced the physical and chemical properties of ABP-CM4 but did not decrease its antibacterial activity. The storage stability and susceptibility to proteinases of ABP-CM4 were apparently improved under the protection of β-CD. This technology could also be widely applied to other AMPs as an antimicrobial system to be used in the food industry.

Keywords: ABP-CM4; Antibacterial assay; Inclusion complex; Stability; β-Cyclodextrin.

MeSH terms

Anti-Bacterial Agents / chemistry
Anti-Bacterial Agents / pharmacology*
Antimicrobial Cationic Peptides / chemistry
Antimicrobial Cationic Peptides / pharmacology*
Bacteria / drug effects*
Fruit / drug effects*
Fruit / microbiology
beta-Cyclodextrins / chemistry*

Substances

Anti-Bacterial Agents
Antimicrobial Cationic Peptides
beta-Cyclodextrins