NS1 antigen: A new beam of light in the early diagnosis of dengue infection

Muhammad Suleman; Rani Faryal; Muhammad Masroor Alam; Salmaan Sharif; Shahzad Shaukat; Uzma Bashir Aamir; Adnan Khurshid; Mehar Angez; Massab Umair; Mian Muhammad Sufian; Yasir Arshad; Ghulam Mujtaba; Syed Sohail Zahoor Zaidi

doi:10.1016/j.apjtm.2016.10.007

NS1 antigen: A new beam of light in the early diagnosis of dengue infection

Asian Pac J Trop Med. 2016 Dec;9(12):1212-1214. doi: 10.1016/j.apjtm.2016.10.007. Epub 2016 Nov 9.

Affiliations

¹ Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan; Department of Virology, National Institute of Health, Park Road, Chak Shahzad, Islamabad, Pakistan.
² Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan.
³ Department of Virology, National Institute of Health, Park Road, Chak Shahzad, Islamabad, Pakistan.
⁴ Department of Virology, National Institute of Health, Park Road, Chak Shahzad, Islamabad, Pakistan. Electronic address: sohailz@live.com.

PMID: 27955749
DOI: 10.1016/j.apjtm.2016.10.007

Abstract

Objective: To evaluate NS1 antigen detection ELISA for the early laboratory diagnosis of dengue virus infection.

Methods: The present study was conducted to evaluate the overall positivity of NS1 antigen detection ELISA and its comparison with viral RNA detection via real time PCR and IgM antibodies detection by ELISA.

Results: A total of 1270 serum samples were tested 86% (1097/1270) were detected positive by one or more than one diagnostic test. Out of 1 270, 64% (807/1270) were positive by NS1 ELISA and 52% (662/1270), 51% (646/1270) were positive by real-time RT-PCR and IgM ELISA respectively.

Conclusions: NS1 antigen detection ELISA is highly suitable diagnostic tools and it also has great value for use in outbreak and epidemic situation.

Keywords: Dengue; ELISA; Epidemiology; NS1 antigen; Pakistan.