Experimental infection of adult Scapharca broughtonii with Ostreid herpesvirus SB strain

Chang-Ming Bai; Qing-Chen Wang; Benjamin Morga; Jie Shi; Chong-Ming Wang

doi:10.1016/j.jip.2016.12.001

Experimental infection of adult Scapharca broughtonii with Ostreid herpesvirus SB strain

J Invertebr Pathol. 2017 Feb:143:79-82. doi: 10.1016/j.jip.2016.12.001. Epub 2016 Dec 8.

Authors

Chang-Ming Bai¹, Qing-Chen Wang², Benjamin Morga³, Jie Shi², Chong-Ming Wang⁴

Affiliations

¹ Division of Maricultural Organism Disease Control and Molecular Pathology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China; Laboratory for Marine Fisheries and Aquaculture, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
² Division of Maricultural Organism Disease Control and Molecular Pathology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China.
³ Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Laboratoire de Génétique et Pathologie (LGP), Avenue de Mus de Loup, 17390 La Tremblade, France.
⁴ Division of Maricultural Organism Disease Control and Molecular Pathology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China; Laboratory for Marine Fisheries and Aquaculture, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China. Electronic address: wangcm@ysfri.ac.cn.

PMID: 27939653
DOI: 10.1016/j.jip.2016.12.001

Abstract

We investigated the susceptibility of ark shell, Scapharca broughtonii, adults to Ostreid herpesvirus SB strain (OsHV-1-SB) through experimental infection by intramuscular injection assays. Results showed the onset of mortality occurred at 3days post injection, one day after the water turbidity became evident in rearing tanks. The mortality curves for the challenged group were similar to those observed at affected hatcheries. Histological lesions, herpesvirus-like particles and high OsHV-1-SB quantities were detected in challenged ark shells. This is the first study to successfully reproduce OsHV-1 disease in Arcoida species, and very few studies in adult bivalves (over 24months old).

Keywords: Experimental infection; OsHV-1-SB; Ostreid herpesvirus SB strain; Scapharca broughtonii; Viral quantity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Herpesviridae / pathogenicity
Herpesviridae Infections / veterinary*
Microscopy, Electron, Transmission
Polymerase Chain Reaction
Scapharca / virology*