Genetic, immunological, and clinical features of patients with bacterial and fungal infections due to inherited IL-17RA deficiency

Proc Natl Acad Sci U S A. 2016 Dec 20;113(51):E8277-E8285. doi: 10.1073/pnas.1618300114. Epub 2016 Dec 7.

Abstract

Chronic mucocutaneous candidiasis (CMC) is defined as recurrent or persistent infection of the skin, nails, and/or mucosae with commensal Candida species. The first genetic etiology of isolated CMC-autosomal recessive (AR) IL-17 receptor A (IL-17RA) deficiency-was reported in 2011, in a single patient. We report here 21 patients with complete AR IL-17RA deficiency, including this first patient. Each patient is homozygous for 1 of 12 different IL-17RA alleles, 8 of which create a premature stop codon upstream from the transmembrane domain and have been predicted and/or shown to prevent expression of the receptor on the surface of circulating leukocytes and dermal fibroblasts. Three other mutant alleles create a premature stop codon downstream from the transmembrane domain, one of which encodes a surface-expressed receptor. Finally, the only known missense allele (p.D387N) also encodes a surface-expressed receptor. All of the alleles tested abolish cellular responses to IL-17A and -17F homodimers and heterodimers in fibroblasts and to IL-17E/IL-25 in leukocytes. The patients are currently aged from 2 to 35 y and originate from 12 unrelated kindreds. All had their first CMC episode by 6 mo of age. Fourteen patients presented various forms of staphylococcal skin disease. Eight were also prone to various bacterial infections of the respiratory tract. Human IL-17RA is, thus, essential for mucocutaneous immunity to Candida and Staphylococcus, but otherwise largely redundant. A diagnosis of AR IL-17RA deficiency should be considered in children or adults with CMC, cutaneous staphylococcal disease, or both, even if IL-17RA is detected on the cell surface.

Keywords: candidiasis; genetics; immunodeficiency.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Bacterial Infections / immunology*
  • Candida
  • Candidiasis / immunology*
  • Cell Membrane
  • Child
  • Child, Preschool
  • Family Health
  • Female
  • Fibroblasts / metabolism
  • Genes, Recessive
  • Genome-Wide Association Study
  • HEK293 Cells
  • Homozygote
  • Humans
  • Immunophenotyping
  • Infant
  • Infant, Newborn
  • Interleukin-17 / metabolism
  • Male
  • Mutation
  • Mycoses / immunology*
  • Open Reading Frames
  • Pedigree
  • Receptors, Interleukin-17 / deficiency*
  • Receptors, Interleukin-17 / genetics*
  • Receptors, Interleukin-17 / metabolism
  • Skin / microbiology
  • T-Lymphocytes / cytology

Substances

  • IL17A protein, human
  • IL17F protein, human
  • IL17RA protein, human
  • IL25 protein, human
  • Interleukin-17
  • Receptors, Interleukin-17