Virus infection drives IL-2 antibody complexes into pro-inflammatory agonists in mice

Sci Rep. 2016 Nov 25:6:37603. doi: 10.1038/srep37603.

Abstract

The use of IL-2/JES6-1 Ab complex (IL-2 Ab Cx) has been considered as a potential therapeutic for inflammatory diseases due to its selective expansion of regulatory T cells (Tregs) in mice. Here, IL-2 Ab Cx was explored as a therapeutic agent to reduce joint inflammation induced by chikungunya virus, an alphavirus causing debilitating joint disease globally. Virus-infected mice treated with IL-2 Ab Cx exhibited exacerbated joint inflammation due to infiltration of highly activated CD4+ effector T cells (Teffs). Virus infection led to upregulation of CD25 on the Teffs, rendering them sensitive towards IL2 Ab Cx. Ready responsiveness of Teffs to IL-2 was further demonstrated in healthy human donors, suggesting that the use of IL-2 Ab Cx in humans is not suitable. Changes in IL-2 sensitivity during active virus infection could change the responsive pattern towards the IL-2 Ab Cx, resulting in the expansion of pro-inflammatory rather than anti-inflammatory responses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies / metabolism*
  • Antigens, CD / metabolism
  • Chikungunya Fever / immunology*
  • Chikungunya Fever / virology*
  • Chikungunya virus / physiology*
  • Female
  • Forkhead Transcription Factors / metabolism
  • Inflammation Mediators / metabolism*
  • Interleukin-2 / administration & dosage
  • Interleukin-2 / metabolism*
  • Interleukin-2 / pharmacology
  • Joints / pathology
  • Joints / virology
  • Lymph Nodes / pathology
  • Mice, Inbred C57BL
  • Mice, Knockout
  • T-Lymphocytes / immunology
  • T-Lymphocytes / metabolism

Substances

  • Antibodies
  • Antigens, CD
  • Forkhead Transcription Factors
  • Foxp3 protein, mouse
  • Inflammation Mediators
  • Interleukin-2