A possible autocrine role for interleukin-6 in two lymphoma cell lines

Blood. 1989 Aug 1;74(2):798-804.

Abstract

Interleukin-6 (IL-6) is a growth factor with diverse biologic activity. Originally described as a T-cell product that enhances immunoglobulin (Ig) secretion in antigen-stimulated B cells, it also affects the growth of T cells, plasmacytomas, hybridomas, and hematopoietic stem cells. We report the expression and secretion of IL-6 by two lymphoma cell lines, OCI-LY3 and OCI-LY12. Addition of recombinant IL-6 stimulated their growth, whereas addition of polyclonal anti-recombinant IL-6 (anti-rIL-6) had a marked inhibitory effect on proliferation. These results suggest an autocrine role for IL-6 in the growth of these lymphoma cells in culture.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Northern
  • Cell Count
  • Cell Division / drug effects
  • Cell Line
  • DNA, Neoplasm / isolation & purification
  • Growth Substances / pharmacology
  • Humans
  • Immune Sera / pharmacology
  • Interleukin-6
  • Interleukins / biosynthesis
  • Interleukins / immunology
  • Interleukins / physiology*
  • Lymphoma / metabolism*
  • Nucleic Acid Hybridization
  • RNA, Messenger / isolation & purification
  • Ribonucleases
  • Tumor Cells, Cultured / metabolism*

Substances

  • DNA, Neoplasm
  • Growth Substances
  • Immune Sera
  • Interleukin-6
  • Interleukins
  • RNA, Messenger
  • Ribonucleases