Mitochondrial Targeting of Metformin Enhances Its Activity against Pancreatic Cancer

Mol Cancer Ther. 2016 Dec;15(12):2875-2886. doi: 10.1158/1535-7163.MCT-15-1021. Epub 2016 Oct 7.

Abstract

Pancreatic cancer is one of the hardest-to-treat types of neoplastic diseases. Metformin, a widely prescribed drug against type 2 diabetes mellitus, is being trialed as an agent against pancreatic cancer, although its efficacy is low. With the idea of delivering metformin to its molecular target, the mitochondrial complex I (CI), we tagged the agent with the mitochondrial vector, triphenylphosphonium group. Mitochondrially targeted metformin (MitoMet) was found to kill a panel of pancreatic cancer cells three to four orders of magnitude more efficiently than found for the parental compound. Respiration assessment documented CI as the molecular target for MitoMet, which was corroborated by molecular modeling. MitoMet also efficiently suppressed pancreatic tumors in three mouse models. We propose that the novel mitochondrially targeted agent is clinically highly intriguing, and it has a potential to greatly improve the bleak prospects of patients with pancreatic cancer. Mol Cancer Ther; 15(12); 2875-86. ©2016 AACR.

MeSH terms

  • Animals
  • Antimetabolites, Antineoplastic / chemistry
  • Antimetabolites, Antineoplastic / pharmacology*
  • Apoptosis / drug effects
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Disease Models, Animal
  • Dose-Response Relationship, Drug
  • Electron Transport Complex I / antagonists & inhibitors
  • Electron Transport Complex I / chemistry
  • Electron Transport Complex I / metabolism
  • Female
  • Humans
  • Hydrogen-Ion Concentration
  • Membrane Potential, Mitochondrial
  • Metformin / chemistry
  • Metformin / pharmacology*
  • Mice
  • Mitochondria / drug effects*
  • Mitochondria / metabolism*
  • Models, Molecular
  • Molecular Conformation
  • Molecular Targeted Therapy
  • Oxygen Consumption
  • Pancreatic Neoplasms / drug therapy
  • Pancreatic Neoplasms / metabolism*
  • Pancreatic Neoplasms / pathology*
  • Protein Binding
  • Rats
  • Reactive Oxygen Species / metabolism
  • Signal Transduction / drug effects
  • Tumor Burden / drug effects
  • Xenograft Model Antitumor Assays

Substances

  • Antimetabolites, Antineoplastic
  • Reactive Oxygen Species
  • Metformin
  • Electron Transport Complex I