Whole Genome Sequence of Two Wild-Derived Mus musculus domesticus Inbred Strains, LEWES/EiJ and ZALENDE/EiJ, with Different Diploid Numbers

G3 (Bethesda). 2016 Dec 7;6(12):4211-4216. doi: 10.1534/g3.116.034751.

Abstract

Wild-derived mouse inbred strains are becoming increasingly popular for complex traits analysis, evolutionary studies, and systems genetics. Here, we report the whole-genome sequencing of two wild-derived mouse inbred strains, LEWES/EiJ and ZALENDE/EiJ, of Mus musculus domesticus origin. These two inbred strains were selected based on their geographic origin, karyotype, and use in ongoing research. We generated 14× and 18× coverage sequence, respectively, and discovered over 1.1 million novel variants, most of which are private to one of these strains. This report expands the number of wild-derived inbred genomes in the Mus genus from six to eight. The sequence variation can be accessed via an online query tool; variant calls (VCF format) and alignments (BAM format) are available for download from a dedicated ftp site. Finally, the sequencing data have also been stored in a lossless, compressed, and indexed format using the multi-string Burrows-Wheeler transform. All data can be used without restriction.

Keywords: Robertsonian translocations; inbred mouse strains; karyotype evolution; wild-derived mouse strains.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Wild / classification
  • Animals, Wild / genetics*
  • Diploidy*
  • Female
  • Genetic Variation
  • Genome*
  • Genomics / methods
  • High-Throughput Nucleotide Sequencing
  • Male
  • Mice
  • Mice, Inbred Strains / classification
  • Mice, Inbred Strains / genetics*
  • Phylogeny