Aerobic glycolysis promotes T helper 1 cell differentiation through an epigenetic mechanism

Science. 2016 Oct 28;354(6311):481-484. doi: 10.1126/science.aaf6284. Epub 2016 Sep 29.

Abstract

Aerobic glycolysis (the Warburg effect) is a metabolic hallmark of activated T cells and has been implicated in augmenting effector T cell responses, including expression of the proinflammatory cytokine interferon-γ (IFN-γ), via 3' untranslated region (3'UTR)-mediated mechanisms. Here, we show that lactate dehydrogenase A (LDHA) is induced in activated T cells to support aerobic glycolysis but promotes IFN-γ expression independently of its 3'UTR. Instead, LDHA maintains high concentrations of acetyl-coenzyme A to enhance histone acetylation and transcription of Ifng Ablation of LDHA in T cells protects mice from immunopathology triggered by excessive IFN-γ expression or deficiency of regulatory T cells. These findings reveal an epigenetic mechanism by which aerobic glycolysis promotes effector T cell differentiation and suggest that LDHA may be targeted therapeutically in autoinflammatory diseases.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • 3' Untranslated Regions
  • Acetylation
  • Aerobiosis
  • Animals
  • Cell Differentiation / genetics*
  • Epigenesis, Genetic*
  • Gene Expression
  • Glycolysis / genetics*
  • Interferon-gamma / genetics
  • Isoenzymes / genetics
  • Isoenzymes / metabolism
  • L-Lactate Dehydrogenase / genetics
  • L-Lactate Dehydrogenase / metabolism*
  • Lactate Dehydrogenase 5
  • Lymphocyte Activation
  • Mice
  • Mice, Transgenic
  • T-Lymphocytes, Regulatory / cytology
  • T-Lymphocytes, Regulatory / enzymology*
  • Th1 Cells / cytology*
  • Th1 Cells / enzymology

Substances

  • 3' Untranslated Regions
  • Isoenzymes
  • Interferon-gamma
  • L-Lactate Dehydrogenase
  • Lactate Dehydrogenase 5