Comparison of two chemiluminescent immunoassays in the detection of measles IgM antibodies

J Virol Methods. 2016 Nov:237:38-39. doi: 10.1016/j.jviromet.2016.08.018. Epub 2016 Aug 26.

Abstract

Serological confirmation of measles is achieved by detecting the specific immunoglobulin M (IgM), and it is important to evaluate new commercial inmunoassays in order to ensure the quality of results. The objective of this study was to compare the performance of a novel automated chemiluminescent immunoassay (CLIA), Virclia IgM measles (Vircell, Spain), with that of the widely used Liaison measles IgM assay (DiaSorin, Italy). A panel of 86 sera from laboratory-confirmed cases was used for the sensitivity calculation, and 59 sera from healthy individuals and those with other viral infections were used for the specificity calculation. Sensitivity values were 96.5% for Virclia and 97.6% for Liaison; specificity values were 93.2% for Virclia and 96.6% for Liaison; neither difference was statistically significant VirClia IgM measles is a good alternative to other immunoassays for the serological confirmation of measles.

Keywords: Chemiluminescent immunoassay; Measles; Serology.

Publication types

  • Comparative Study

MeSH terms

  • Antibodies, Viral / blood*
  • Humans
  • Immunoassay / methods*
  • Immunoassay / standards
  • Immunoenzyme Techniques / methods
  • Immunoglobulin G / blood
  • Immunoglobulin M / blood*
  • Italy
  • Luminescent Measurements*
  • Measles / diagnosis*
  • Measles / immunology*
  • Measles / virology
  • Measles virus / immunology*
  • Measles virus / isolation & purification
  • Reagent Kits, Diagnostic
  • Sensitivity and Specificity
  • Serologic Tests / methods
  • Spain

Substances

  • Antibodies, Viral
  • Immunoglobulin G
  • Immunoglobulin M
  • Reagent Kits, Diagnostic