Predominant prevalence of human rotaviruses with the G1P[8] and G8P[8] genotypes with a short RNA profile in 2013 and 2014 in Sukhothai and Phetchaboon provinces, Thailand

J Med Virol. 2017 Apr;89(4):615-620. doi: 10.1002/jmv.24669. Epub 2016 Sep 7.

Abstract

Of 2,754 stool specimens collected from children with acute gastroenteritis during 2013-2014 in Sukhothai and Phetchaboon provinces, Thailand, 666 (24.2%) were positive for rotavirus A (RVA) in polyacrylamide gel electrophoresis (PAGE). The G and P types of all RVA-positive specimens were determined by semi-nested RT-PCR. G1P[8] (56.5%) was most prevalent, followed by G2P[4] (22.1%). Unusual G8P[8] human RVAs (HuRVAs) were detected at a high frequency (20.0%). Interestingly, 171 of the 376 G1P[8] HuRVAs and all of the 133 G8P[8] HuRVAs showed a short RNA pattern in PAGE. Thus, it was shown that the properties of HuRVAs have been markedly unusual in recent years in Thailand. J. Med. Virol. 89:615-620, 2017. © 2016 Wiley Periodicals, Inc.

Keywords: epidemiology; gastroenteritis; genotype; polyacrylamide gel electrophoresis; rotavirus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Child
  • Child, Preschool
  • Electrophoresis, Polyacrylamide Gel
  • Feces / virology
  • Female
  • Genotype*
  • Humans
  • Infant
  • Infant, Newborn
  • Male
  • Molecular Epidemiology
  • Polymerase Chain Reaction
  • Prevalence
  • RNA, Viral / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Rotavirus / classification*
  • Rotavirus / genetics*
  • Rotavirus / isolation & purification
  • Rotavirus Infections / epidemiology*
  • Rotavirus Infections / virology*
  • Thailand / epidemiology

Substances

  • RNA, Viral