OMIP-035: Functional analysis of natural killer cell subsets in macaques

Cytometry A. 2016 Sep;89(9):799-802. doi: 10.1002/cyto.a.22932. Epub 2016 Aug 17.

Abstract

This panel was developed to measure the functional capability of natural killer (NK) cell subsets in rhesus macaques (Macaca mulatta). It includes markers to determine the frequency of cytokine secreting and cytotoxic NK cell subpopulations in peripheral blood mononuclear cell (PBMC) samples stimulated in vitro with human 721.221 cells. NK cell subsets were defined by the expression of killer cell immunoglobulin-like receptors (KIRs) Mamu-KIR3DL01 and Mamu-KIR3DL05, and differentiation antigens CD16 and CD56. The panel can be used to assess the functional capability of NK cells in a range of normal and pathologic conditions of captive bred rhesus macaques of Indian origin. © 2016 International Society for Advancement of Cytometry.

Keywords: KIR3DL01; KIR3DL05; NK cell functions; rhesus macaque.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • CD56 Antigen / immunology
  • Epitopes, T-Lymphocyte / immunology*
  • Humans
  • Killer Cells, Natural / cytology*
  • Killer Cells, Natural / immunology
  • Leukocytes, Mononuclear / immunology
  • Macaca mulatta / blood*
  • Macaca mulatta / immunology
  • Receptors, IgG / immunology
  • Receptors, KIR / biosynthesis
  • Receptors, KIR / immunology*

Substances

  • CD56 Antigen
  • Epitopes, T-Lymphocyte
  • Receptors, IgG
  • Receptors, KIR