Towards a multi protein and mRNA expression of biological predictive and distinguish model for post stroke depression

Oncotarget. 2016 Aug 23;7(34):54329-54338. doi: 10.18632/oncotarget.11105.

Abstract

Previous studies suggest that neurotrophic factors participate in the development of stroke and depression. So we investigated the utility of these biomarkers as predictive and distinguish model for post stroke depression (PSD). 159 individuals including PSD, stroke without depression (Non-PSD), major depressive disorder (MDD) and normal control groups were recruited and examined the protein and mRNA expression levels of vascular endothelial growth factor (VEGF), vascular endothelial growth factor receptors (VEGFR2), placental growth factor (PIGF), insulin-like growth factor (IGF-1) and insulin-like growth factor receptors (IGF-1R). The chi-square test was used to evaluate categorical variable, while nonparametric test and one-way analysis of variance were applied to continuous variables of general characteristics, clinical and biological changes. In order to explore the predictive and distinguish role of these factors in PSD, discriminant analysis and receiver operating characteristic curve were calculated. The four groups had statistical differences in these neurotrophic factors (all P < 0.05) except VEGF concentration and IGF-1R mRNA (P = 0.776, P = 0.102 respectively). We identified these mRNA expression and protein analytes with general predictive performance for PSD and Non-PSD groups [area under the curve (AUC): 0.805, 95% CI, 0.704-0.907, P < 0.001]. Importantly, there is an excellent predictive performance (AUC: 0.984, 95% CI, 0.964-1.000, P < 0.001) to differentiate PSD patients from MDD patients. This was the first study to explore the changes of neurotrophic factors family in PSD patients, the results intriguingly demonstrated that the combination of protein and mRNA expression of biological factors could use as a predictive and discriminant model for PSD.

Keywords: Pathology Section; mRNA; neurotrophic factors; post stroke depression; protein.

MeSH terms

  • Aged
  • Depression / metabolism*
  • Depressive Disorder, Major / metabolism
  • Female
  • Humans
  • Insulin-Like Growth Factor I / analysis
  • Insulin-Like Growth Factor I / genetics
  • Male
  • Membrane Proteins / analysis
  • Membrane Proteins / genetics
  • Middle Aged
  • RNA, Messenger / analysis
  • Receptor, IGF Type 1
  • Receptors, Somatomedin / analysis
  • Receptors, Somatomedin / genetics
  • Stroke / psychology*
  • Vascular Endothelial Growth Factor Receptor-2 / analysis
  • Vascular Endothelial Growth Factor Receptor-2 / genetics

Substances

  • IGF1 protein, human
  • IGF1R protein, human
  • Membrane Proteins
  • PIGF protein, human
  • RNA, Messenger
  • Receptors, Somatomedin
  • Insulin-Like Growth Factor I
  • Receptor, IGF Type 1
  • Vascular Endothelial Growth Factor Receptor-2