Overexpression of DCLK1 is predictive for recurrent disease in major salivary gland malignancies

Eur Arch Otorhinolaryngol. 2017 Jan;274(1):467-475. doi: 10.1007/s00405-016-4227-7. Epub 2016 Jul 28.

Abstract

Salivary gland carcinomas are a rare malignancy. Therefore, little is known about biomarkers and cancer stem cells in salivary gland malignancies. Double cortin-like kinase 1 (DCLK1) is a promising therapeutic target and cancer stem cell marker, predominantly investigated in pancreatic and colorectal cancer. The purpose of this study was to investigate the expression of DCLK1 in major and minor salivary gland carcinomas and its influence on survival. We examined a total of 80 patients with major or minor salivary gland cancer in this retrospective study. Immunohistochemistry with anti-DCLK1 antibody was applied to assess the expression of DCLK1. Moreover, we evaluated the impact of DCLK1 on overall and disease-free survival. DCLK1 expression could be detected in 66.3 % of all examined cases. Overexpression of DCLK1 was associated with reduced overall and disease-free survival in patients with major salivary gland cancer. Disease-free survival reached statistical significance (p = 0.0107). However, expression of DCLK1 had no influence on survival in patients with minor salivary gland cancer. Since treatment of recurrent disease in oncologic patients is utterly challenging, DCLK1 may be a promising prognostic biomarker that helps to identify patients with a high risk for recurrence of major salivary gland carcinoma.

Keywords: Cancer stem cell; DCLK1; Major salivary gland cancer; Minor salivary gland cancer; Molecular prognostic marker.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers, Tumor / metabolism*
  • Carcinoma / metabolism
  • Carcinoma / mortality
  • Carcinoma / pathology
  • Disease-Free Survival
  • Doublecortin-Like Kinases
  • Female
  • Humans
  • Immunohistochemistry
  • Intracellular Signaling Peptides and Proteins / metabolism*
  • Lymphatic Metastasis
  • Male
  • Middle Aged
  • Neoplasm Recurrence, Local / metabolism*
  • Neoplasm Recurrence, Local / pathology
  • Prognosis
  • Protein Serine-Threonine Kinases / metabolism*
  • Retrospective Studies
  • Salivary Gland Neoplasms / metabolism*
  • Salivary Gland Neoplasms / mortality
  • Salivary Gland Neoplasms / pathology
  • Young Adult

Substances

  • Biomarkers, Tumor
  • Intracellular Signaling Peptides and Proteins
  • DCLK1 protein, human
  • Doublecortin-Like Kinases
  • Protein Serine-Threonine Kinases