In the Goodpasture experiment, we determined a probability of 99.1% of identifying one single immune complex. Under these conditions, a single molecule event is proven. There exist no instrumental assumptions of our approach on which the experiments themselves, the theoretical background or the conclusion are based on. Our results open up a broad field for analytics and diagnostics in solution, particularly in immunology and immunohematology.
Keywords: Critical bulk concentration; Fluorescence correlation spectroscopy; Goodpasture autoantibodies; Poisson distribution; Single immune complex; Solution-phase single-molecule fluorescence correlation spectroscopy.