Biosynthesis of ZnO nanoparticles using Jacaranda mimosifolia flowers extract: Synergistic antibacterial activity and molecular simulated facet specific adsorption studies

Deepali Sharma; Myalowenkosi I Sabela; Suvardhan Kanchi; Phumlane S Mdluli; Gulshan Singh; Thor A Stenström; Krishna Bisetty

doi:10.1016/j.jphotobiol.2016.06.043

Biosynthesis of ZnO nanoparticles using Jacaranda mimosifolia flowers extract: Synergistic antibacterial activity and molecular simulated facet specific adsorption studies

J Photochem Photobiol B. 2016 Sep:162:199-207. doi: 10.1016/j.jphotobiol.2016.06.043. Epub 2016 Jun 25.

Authors

Deepali Sharma¹, Myalowenkosi I Sabela², Suvardhan Kanchi³, Phumlane S Mdluli², Gulshan Singh⁴, Thor A Stenström⁴, Krishna Bisetty⁵

Affiliations

¹ Department of Chemistry, Durban University of Technology, P.O. Box 1334, Durban 4000, South Africa. Electronic address: dpschem@gmail.com.
² Department of Chemistry, Durban University of Technology, P.O. Box 1334, Durban 4000, South Africa.
³ Department of Chemistry, Durban University of Technology, P.O. Box 1334, Durban 4000, South Africa. Electronic address: ksuvardhan@gmail.com.
⁴ SARChi Chair, Institute for Water and Wastewater Technology, Durban University of Technology, P.O. Box 1334, Durban 4000, South Africa.
⁵ Department of Chemistry, Durban University of Technology, P.O. Box 1334, Durban 4000, South Africa. Electronic address: bisettk@dut.ac.za.

PMID: 27380295
DOI: 10.1016/j.jphotobiol.2016.06.043

Abstract

The naturally occurring biomolecules present in the plant extracts have been identified to play an active role in the single step formation of nanoparticles with varied morphologies and sizes which is greener and environmentally benign. In the present work, spherical zinc oxide nanoparticles (ZnO NPs) of 2-4nm size were synthesized using aqueous extract of fallen Jacaranda mimosifolia flowers (JMFs), treated as waste. The microwave assisted synthesis was completed successfully within 5min. Thereafter, phase identification, morphology and optical band gap of the synthesized ZnO NPs were done using X-ray diffraction (XRD), high resolution transmission electron microscopy (HRTEM) and UV-Visible spectroscopy techniques. The composition of JMFs extract was analyzed by gas chromatography-mass spectrometry (GC-MS) and the ZnO NPs confirmation was further explored with fourier transform infrared spectroscopy (FTIR). The GC-MS results confirmed the presence of oleic acid which has high propensity of acting as a reducing and capping agent. The UV-Visible data suggested an optical band gap of 4.03eV for ZnO NPs indicating their small size due to quantum confinement. Further, facet specific adsorption of oleic acid on the surface of ZnO NPs was studied computationally to find out the impact of biomolecules in defining the shape and size of NPs. The viability of gram negative Escherichia coli and gram positive Enterococcus faecium bacteria was found to be 48% and 43%, respectively at high concentration of NPs.

Keywords: Antibacterial activity; Biosynthesis; Jacaranda mimosifolia flowers extract; Zinc oxide nanoparticles.

MeSH terms

Adsorption
Anti-Bacterial Agents / chemical synthesis
Anti-Bacterial Agents / chemistry
Anti-Bacterial Agents / pharmacology
Bignoniaceae / chemistry*
Chemistry Techniques, Synthetic
Escherichia coli / drug effects
Flowers / chemistry*
Models, Molecular
Nanoparticles*
Nanotechnology*
Particle Size
Plant Extracts / chemistry*
Zinc Oxide / chemical synthesis
Zinc Oxide / chemistry*
Zinc Oxide / pharmacology*

Substances

Anti-Bacterial Agents
Plant Extracts
Zinc Oxide