Transcription Factor NFIB Is a Driver of Small Cell Lung Cancer Progression in Mice and Marks Metastatic Disease in Patients

Cell Rep. 2016 Jul 19;16(3):631-43. doi: 10.1016/j.celrep.2016.06.020. Epub 2016 Jun 30.

Abstract

Small cell lung cancer (SCLC) is an aggressive neuroendocrine tumor, and no effective treatment is available to date. Mouse models of SCLC based on the inactivation of Rb1 and Trp53 show frequent amplifications of the Nfib and Mycl genes. Here, we report that, although overexpression of either transcription factor accelerates tumor growth, NFIB specifically promotes metastatic spread. High NFIB levels are associated with expansive growth of a poorly differentiated and almost exclusively E-cadherin (CDH1)-negative invasive tumor cell population. Consistent with the mouse data, we find that NFIB is overexpressed in almost all tested human metastatic high-grade neuroendocrine lung tumors, warranting further assessment of NFIB as a tumor progression marker in a clinical setting.

MeSH terms

  • Animals
  • Biomarkers, Tumor / metabolism*
  • Cadherins / metabolism
  • Cell Proliferation / physiology
  • Disease Models, Animal
  • Disease Progression
  • Gene Expression Regulation, Neoplastic / physiology
  • Humans
  • Lung Neoplasms / metabolism*
  • Lung Neoplasms / pathology*
  • Mice
  • NFI Transcription Factors / metabolism*
  • Neoplasm Metastasis / pathology
  • Proto-Oncogene Proteins c-myc / metabolism
  • Retinoblastoma Protein / metabolism
  • Small Cell Lung Carcinoma / metabolism*
  • Small Cell Lung Carcinoma / pathology*
  • Tumor Suppressor Protein p53 / metabolism

Substances

  • Biomarkers, Tumor
  • Cadherins
  • NFI Transcription Factors
  • NFIB protein, human
  • Nfib protein, mouse
  • Proto-Oncogene Proteins c-myc
  • Retinoblastoma Protein
  • Tumor Suppressor Protein p53