Regioisomeric and enantiomeric analysis of triacylglycerols

Tomáš Řezanka; Karolína Pádrová; Karel Sigler

doi:10.1016/j.ab.2016.05.028

Regioisomeric and enantiomeric analysis of triacylglycerols

Anal Biochem. 2017 May 1:524:3-12. doi: 10.1016/j.ab.2016.05.028. Epub 2016 Jun 16.

Authors

Tomáš Řezanka¹, Karolína Pádrová², Karel Sigler³

Affiliations

¹ Institute of Microbiology, CAS, Vídeňská 1083, 142 20, Prague, Czech Republic. Electronic address: rezanka@biomed.cas.cz.
² Department of Biotechnology, University of Chemistry and Technology, Prague, Technická 5, 166 28, Prague, Czech Republic.
³ Institute of Microbiology, CAS, Vídeňská 1083, 142 20, Prague, Czech Republic.

PMID: 27318242
DOI: 10.1016/j.ab.2016.05.028

Abstract

A survey of useful methods for separation and identification of regioisomers and enantiomers of triacylglycerols. Gas chromatography, gas chromatography-mass spectrometry, ¹³C NMR determination of regioisomers by enzymatic methods, and supercritical fluid chromatography are briefly surveyed, whereas a detailed description is given of the analysis of triacylglycerols by liquid chromatography, especially with silver ion (Ag⁺; argentation), and nonaqueous reversed phase liquid chromatography. Special attention is paid to chiral chromatography. Details of mass spectrometry of triacylglycerols are also described, especially the identification of important triacylglycerol ions such as [M + H-RCOOH]⁺ in atmospheric pressure chemical ionization mass spectra.

Keywords: Atmospheric pressure chemical ionization; Enantiomers; High-performance liquid chromatography; Regioisomers; Triacylglycerols.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gas Chromatography-Mass Spectrometry / methods*
Silver / chemistry
Triglycerides / analysis*

Substances

Triglycerides
Silver