sesC as a genetic marker for easy identification of Staphylococcus epidermidis from other isolates

Ladan Khodaparast; Laleh Khodaparast; Lieve Van Mellaert; Mohammad Shahrooei; Marc Van Ranst; Johan Van Eldere

doi:10.1016/j.meegid.2016.05.037

sesC as a genetic marker for easy identification of Staphylococcus epidermidis from other isolates

Infect Genet Evol. 2016 Sep:43:222-4. doi: 10.1016/j.meegid.2016.05.037. Epub 2016 May 31.

Authors

Ladan Khodaparast¹, Laleh Khodaparast², Lieve Van Mellaert³, Mohammad Shahrooei⁴, Marc Van Ranst⁵, Johan Van Eldere⁶

Affiliations

¹ KU Leuven, University of Leuven, Department of Microbiology and Immunology, Clinical Bacteriology and Mycology, Herestraat 49, box 819, B-3000 Leuven, Belgium; KU Leuven, University of Leuven, Department of Cellular and Molecular Medicine, Switch Laboratory, Herestraat 49, box 802, B-3000 Leuven, Belgium.
² KU Leuven, University of Leuven, Department of Microbiology and Immunology, Clinical Bacteriology and Mycology, Herestraat 49, box 819, B-3000 Leuven, Belgium; KU Leuven, University of Leuven, Department of Cellular and Molecular Medicine, Switch Laboratory, Herestraat 49, box 802, B-3000 Leuven, Belgium. Electronic address: laleh.khodaparast@switch.vib-kuleuven.be.
³ KU Leuven, University of Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Molecular Bacteriology, Herestraat 49, box 1037, B-3000 Leuven, Belgium.
⁴ KU Leuven, University of Leuven, Department of Microbiology and Immunology, Clinical Bacteriology and Mycology, Herestraat 49, box 819, B-3000 Leuven, Belgium.
⁵ KU Leuven, University of Leuven, University Hospitals Leuven, Department of Microbiology and Immunology, Herestraat 49, box 7001, B-3000 Leuven, Belgium; KU Leuven, University of Leuven, University Hospitals Leuven, Department of Microbiology and Immunology, Kapucijnenvoer 33 blok I, box 7001, B-3000 Leuven, Belgium.
⁶ KU Leuven, University of Leuven, Department of Microbiology and Immunology, Clinical Bacteriology and Mycology, Herestraat 49, box 819, B-3000 Leuven, Belgium; KU Leuven, University of Leuven, University Hospitals Leuven, Department of Laboratory Medicine, Herestraat 49, box 7003, B-3000 Leuven, Belgium. Electronic address: johan.vaneldere@uzleuven.be.

PMID: 27259364
DOI: 10.1016/j.meegid.2016.05.037

Abstract

Staphylococcus epidermidis is one of the major concerns with respect to hospital-acquired infections. Therefore, a rapid and easy method to identify at species level S. epidermidis isolates out of a broad range of bacteria is necessary. Based on earlier studies, the sesC gene encoding a S. epidermidis surface protein revealed to be a highly conserved gene in this species. By means of an easy and inexpensive PCR assay, the presence of sesC was checked in 438 clinical staphylococcal isolates. Results showed that sesC is specifically present in all S. epidermidis. In conclusion, the sesC gene can be exploited as a genetic marker in order to distinguish S. epidermidis from other isolates.

Keywords: Rapid and easy identification; Staphylococcus epidermidis; sesC.

MeSH terms

Bacterial Proteins / genetics*
Bacterial Typing Techniques
Cross Infection / diagnosis*
Cross Infection / microbiology
DNA Primers / chemistry
Gene Expression
Genetic Markers
Humans
Membrane Proteins / genetics*
Polymerase Chain Reaction / methods
Staphylococcal Infections / diagnosis*
Staphylococcal Infections / microbiology
Staphylococcus epidermidis / classification
Staphylococcus epidermidis / genetics*
Staphylococcus epidermidis / isolation & purification

Substances

Bacterial Proteins
DNA Primers
Genetic Markers
Membrane Proteins