Piceatannol increases the expression of hepatocyte growth factor and IL-10 thereby protecting hepatocytes in thioacetamide-induced liver fibrosis

Can J Physiol Pharmacol. 2016 Jul;94(7):779-87. doi: 10.1139/cjpp-2016-0001. Epub 2016 Mar 5.

Abstract

Piceatannol is a polyphenolic analog of resveratrol that selectively inhibits the non-receptor tyrosine kinase-Syk. This study investigates the potential ability of piceatannol to attenuate liver fibrosis and protect hepatocytes from injury. Thioacetamide was injected in adult male mice (100 mg/kg, i.p., 3 times/week) for 8 weeks. Piceatannol (1 or 5 mg/kg per day) was administered by oral gavage during the last 4 weeks. Liver function biomarkers, tissue malondialdehyde (MDA), cytokeratin-18 (CK18), hepatocyte growth factor (HGF), and interleukin-10 (IL-10) were measured. Necroinflammation, fibrosis, expression of transforming growth factor (TGF)-β1, and α-smooth muscle actin (SMA) were scored by histopathological examination and immunohistochemistry. Obtained results showed ability of piceatannol (1 mg/kg) to restore liver function and reduce inflammation. It significantly (p < 0.001) reduced MDA, CK18, TGF-β1, and α-SMA expression, and increased HGF and IL-10. It can be concluded that piceatannol at low dose can inhibit TGF-β1 induced hepatocytes apoptosis and exerts an anti-inflammatory effect attenuating fibrosis progression.

Keywords: HGF; IL-10; cytokeratin-18; cytokératine-18; hepatocytes; piceatannol.

MeSH terms

  • Animals
  • Gene Expression Regulation
  • Hepatocyte Growth Factor / biosynthesis*
  • Hepatocyte Growth Factor / genetics
  • Hepatocytes / drug effects
  • Hepatocytes / metabolism*
  • Interleukin-10 / biosynthesis*
  • Interleukin-10 / genetics
  • Liver Cirrhosis / chemically induced
  • Liver Cirrhosis / metabolism*
  • Liver Cirrhosis / prevention & control
  • Male
  • Mice
  • Protective Agents / pharmacology
  • Protective Agents / therapeutic use
  • Stilbenes / pharmacology
  • Stilbenes / therapeutic use*
  • Thioacetamide / toxicity*

Substances

  • HGF protein, mouse
  • IL10 protein, mouse
  • Protective Agents
  • Stilbenes
  • Thioacetamide
  • Interleukin-10
  • Hepatocyte Growth Factor
  • 3,3',4,5'-tetrahydroxystilbene