Automated synthesis of [(18)F](2S,4R)-4-fluoroglutamine on a GE TRACERlab™ FX-N Pro module

Appl Radiat Isot. 2016 Jun:112:110-4. doi: 10.1016/j.apradiso.2016.02.016. Epub 2016 Mar 17.

Abstract

Glutamine (Gln) and its analogues may serve as imaging agents for tumor diagnosis using positron emission tomography (PET), especially for tumors with negative [(18)F]FDG scan. We report the first automated synthesis of [(18)F](2S,4R)-4-fluoroglutamine ([(18)F]FGln) on a GE TRACERlab™ FX-N Pro module. [(18)F]FGln was obtained in 80±3min with a radiochemical yield of 21±3% (n=5, uncorrected). The radiochemical purity was >98%, and optical purity 90±5%. The synthesis is highly reproducible with good chemical purity, radiochemical yield, and is suitable for translation to cGMP production.

Keywords: Automation; F-18; Fluoroglutamine; PET imaging; Tumor.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural

MeSH terms

  • Automation / instrumentation
  • Automation / methods
  • Chemistry Techniques, Synthetic / instrumentation
  • Chemistry Techniques, Synthetic / methods
  • Drug Stability
  • Fluorine Radioisotopes*
  • Glutamine / analogs & derivatives*
  • Glutamine / chemical synthesis
  • Humans
  • Neoplasms / diagnostic imaging
  • Positron-Emission Tomography / methods
  • Radiopharmaceuticals / chemical synthesis*
  • Technology, Radiologic / instrumentation
  • Technology, Radiologic / methods

Substances

  • 4-fluoroglutamine
  • Fluorine Radioisotopes
  • Radiopharmaceuticals
  • Glutamine