Point of care testing of phospholipase A2 group IIA for serological diagnosis of rheumatoid arthritis

Nanoscale. 2016 Feb 28;8(8):4482-5. doi: 10.1039/c5nr08423g.

Abstract

Secretory phospholipase A2 group IIA (sPLA2-IIA) was examined as a point of care marker for determining disease activity in rheumatoid (RA) and psoriatic (PsA) arthritis. Serum concentration and activity of sPLA2-IIA were measured using in-house antibodies and a novel point of care lateral flow device assay in patients diagnosed with varying severities of RA (n = 30) and PsA (n = 25) and found to correlate strongly with C-reactive protein (CRP). Levels of all markers were elevated in patients with active RA over those with inactive RA as well as both active and inactive PsA, indicating that sPLA2-IIA can be used as an analogue to CRP for RA diagnosis at point of care.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Arthritis, Psoriatic / diagnosis
  • Arthritis, Rheumatoid / diagnosis*
  • Biomarkers / blood
  • C-Reactive Protein
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Group II Phospholipases A2 / blood*
  • Humans
  • Male
  • Middle Aged
  • Point-of-Care Testing

Substances

  • Biomarkers
  • C-Reactive Protein
  • Group II Phospholipases A2
  • PLA2G2A protein, human