Two subunits of mannose permease, II-PMan and II-MMan, of Escherichia coli mediate coliphage N4 infection

Biochem Int. 1989 Mar;18(3):545-9.

Abstract

Mutant strains of Escherichia coli that lack one or all of the intact components of mannose permease do not support the growth of phage N4. Complementation experiments using three recombinant plasmids containing DNA fragments coding for the subunits of mannose permease revealed that among the three component subunits, II-PMan and II-MMan alone are sufficient to confer N4 sensitivity.

Publication types

  • Comparative Study

MeSH terms

  • Coliphages / genetics*
  • Coliphages / pathogenicity
  • DNA, Recombinant
  • Escherichia coli / enzymology
  • Escherichia coli / genetics*
  • Genetic Code
  • Genetic Complementation Test
  • Mutation
  • Phosphoenolpyruvate Sugar Phosphotransferase System / genetics*
  • Phosphoenolpyruvate Sugar Phosphotransferase System / metabolism
  • Plasmids
  • Recombinant Proteins / metabolism
  • Suppression, Genetic

Substances

  • DNA, Recombinant
  • Recombinant Proteins
  • Phosphoenolpyruvate Sugar Phosphotransferase System
  • phosphoenolpyruvate-mannose phosphotransferase