Rapid Detection of OXA-48-Producing Enterobacteriaceae by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

Marina Oviaño; Maria José Barba; Begoña Fernández; Adriana Ortega; Belén Aracil; Jesús Oteo; José Campos; Germán Bou

doi:10.1128/JCM.02496-15

Rapid Detection of OXA-48-Producing Enterobacteriaceae by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

J Clin Microbiol. 2016 Mar;54(3):754-9. doi: 10.1128/JCM.02496-15. Epub 2015 Dec 16.

Authors

Marina Oviaño¹, Maria José Barba¹, Begoña Fernández¹, Adriana Ortega², Belén Aracil², Jesús Oteo², José Campos², Germán Bou³

Affiliations

¹ Servicio de Microbiología, Complejo Hospitalario Universitario A Coruña, La Coruña, Spain.
² Laboratorio de Antibióticos, Bacteriología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain Red Española de Investigación en Patología Infecciosa (REIPI), Madrid, Spain.
³ Servicio de Microbiología, Complejo Hospitalario Universitario A Coruña, La Coruña, Spain Red Española de Investigación en Patología Infecciosa (REIPI), Madrid, Spain german.bou.arevalo@sergas.es.

Abstract

A rapid and sensitive (100%) matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) assay was developed to detect OXA-48-type producers, using 161 previously characterized clinical isolates. Ertapenem was monitored to detect carbapenem resistance, and temocillin was included in the assay as a marker for OXA-48-producers. Structural analysis of temocillin is described. Data are obtained within 60 min.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology
Bacterial Typing Techniques*
Enterobacteriaceae / classification*
Enterobacteriaceae / drug effects
Enterobacteriaceae / enzymology*
Ertapenem
Humans
Sensitivity and Specificity
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization* / methods
beta-Lactamases / biosynthesis*
beta-Lactams / pharmacology

Substances

Anti-Bacterial Agents
beta-Lactams
beta-Lactamases
Ertapenem

Grants and funding

This work was supported by grants from the following organizations: Fondo de Investigación Sanitaria (grants PI12/01242 to J.O. and PI12/00512 to G.B.); the Antibiotic Resistance Surveillance Programme of the Spanish Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ministerio de Economía y Competitividad; the Plan Nacional de I+D+I 2008–2011; and the Instituto de Salud Carlos III, Subdirección General de Redes y Centros de Investigación Cooperativa, Ministerio de Economía y Competitividad, Spanish Network for Research in Infectious Diseases (REIPI RD12/0015), cofinanced by European Development Regional Fund “A Way to Achieve Europe” ERDF. The funders had no role in the study design, data collection, interpretation, or the decision to submit the work for publication.