Implementation of a Consensus Set of Hypervariable Mycobacterial Interspersed Repetitive-Unit-Variable-Number Tandem-Repeat Loci in Mycobacterium tuberculosis Molecular Epidemiology

J Clin Microbiol. 2016 Feb;54(2):478-82. doi: 10.1128/JCM.02945-15. Epub 2015 Dec 9.

Abstract

This study shows that the addition of a consensus 4-locus set of hypervariable mycobacterial interspersed repetitive-unit-variable-number tandem repeat (MIRU-VNTR) loci to the spoligotyping-24-locus MIRU-VNTR typing strategy is a well-standardized approach that can contribute to an improvement of the true cluster definition while retaining high typeability in non-Beijing strains.

MeSH terms

  • Bacterial Typing Techniques
  • Cluster Analysis
  • Genetic Loci*
  • Genetic Markers
  • Genotype
  • Humans
  • Minisatellite Repeats*
  • Molecular Epidemiology
  • Mycobacterium tuberculosis / classification*
  • Mycobacterium tuberculosis / genetics*
  • Polymorphism, Restriction Fragment Length
  • Reagent Kits, Diagnostic
  • Tuberculosis / epidemiology*
  • Tuberculosis / microbiology*

Substances

  • Genetic Markers
  • Reagent Kits, Diagnostic