Identification and characterization of latency-associated peptide-expressing γδ T cells

Nat Commun. 2015 Dec 8:6:8726. doi: 10.1038/ncomms9726.

Abstract

γδ T cells are a subset of lymphocytes specialized in protecting the host against pathogens and tumours. Here we describe a subset of regulatory γδ T cells that express the latency-associated peptide (LAP), a membrane-bound TGF-β1. Thymic CD27+IFN-γ+CCR9+α4β7+TCRγδ+ cells migrate to the periphery, particularly to Peyer's patches and small intestine lamina propria, where they upregulate LAP, downregulate IFN-γ via ATF-3 expression and acquire a regulatory phenotype. TCRγδ+LAP+ cells express antigen presentation molecules and function as antigen presenting cells that induce CD4+Foxp3+ regulatory T cells, although TCRγδ+LAP+ cells do not themselves express Foxp3. Identification of TCRγδ+LAP+ regulatory cells provides an avenue for understanding immune regulation and biologic processes linked to intestinal function and disease.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Activating Transcription Factor 3 / genetics
  • Activating Transcription Factor 3 / immunology
  • Adult
  • Animals
  • Animals, Congenic
  • Antigen-Presenting Cells
  • Colitis / immunology*
  • Cytokines / genetics
  • Cytokines / immunology*
  • Disease Models, Animal
  • Flow Cytometry
  • Forkhead Transcription Factors / genetics
  • Forkhead Transcription Factors / immunology
  • Humans
  • In Vitro Techniques
  • Interferon-gamma
  • Intestinal Mucosa / immunology*
  • Leukocytes, Mononuclear / immunology
  • Mice
  • Peyer's Patches / immunology*
  • Real-Time Polymerase Chain Reaction
  • Receptors, Antigen, T-Cell / immunology
  • Receptors, Antigen, T-Cell, gamma-delta / immunology*
  • Reverse Transcriptase Polymerase Chain Reaction
  • T-Lymphocyte Subsets / immunology*
  • T-Lymphocytes, Regulatory / immunology*
  • Transforming Growth Factor beta1 / genetics
  • Transforming Growth Factor beta1 / immunology*

Substances

  • Activating Transcription Factor 3
  • Atf3 protein, mouse
  • Cytokines
  • Forkhead Transcription Factors
  • Foxp3 protein, mouse
  • Receptors, Antigen, T-Cell
  • Receptors, Antigen, T-Cell, gamma-delta
  • Transforming Growth Factor beta1
  • Interferon-gamma

Associated data

  • GEO/GSE73077