Extended-spectrum β-lactamase (ESBL) detection directly from urine samples with the rapid isothermal amplification-based eazyplex® SuperBug CRE assay: Proof of concept

V Hinić; J Ziegler; C Straub; D Goldenberger; R Frei

doi:10.1016/j.mimet.2015.10.015

Extended-spectrum β-lactamase (ESBL) detection directly from urine samples with the rapid isothermal amplification-based eazyplex® SuperBug CRE assay: Proof of concept

J Microbiol Methods. 2015 Dec:119:203-5. doi: 10.1016/j.mimet.2015.10.015. Epub 2015 Oct 23.

Authors

V Hinić¹, J Ziegler², C Straub², D Goldenberger², R Frei²

Affiliations

¹ Division of Clinical Microbiology, University Hospital Basel, Petersgraben 4, 4031, Basel, Switzerland. Electronic address: vladimira.hinic@usb.ch.
² Division of Clinical Microbiology, University Hospital Basel, Petersgraben 4, 4031, Basel, Switzerland.

PMID: 26506282
DOI: 10.1016/j.mimet.2015.10.015

Abstract

A commercially available assay (eazyplex® SuperBug CRE) detecting the most common carbapenemase and ESBL types was evaluated directly on 50 urine samples. Eazyplex® correctly detected ESBL-encoding genes in all 30 urine samples with confirmed ESBL production (sensitivity 100%). Two specimens showed invalid and one specimen false-positive results (specificity 97.9%).

Keywords: ESBL; Isothermal amplification; Proof of concept; Rapid detection; Urine samples.

Publication types

Evaluation Study

MeSH terms

Bacterial Proteins / genetics
Bacterial Proteins / urine*
Enterobacteriaceae / enzymology*
Enterobacteriaceae / genetics
Enterobacteriaceae / isolation & purification
Enterobacteriaceae Infections / microbiology
Enterobacteriaceae Infections / urine*
Humans
Nucleic Acid Amplification Techniques / economics
Nucleic Acid Amplification Techniques / methods*
Reagent Kits, Diagnostic
beta-Lactamases / genetics
beta-Lactamases / urine*

Substances

Bacterial Proteins
Reagent Kits, Diagnostic
beta-Lactamases
carbapenemase