Opsin evolution in the Ambulacraria

S D'Aniello; J Delroisse; A Valero-Gracia; E K Lowe; M Byrne; J T Cannon; K M Halanych; M R Elphick; J Mallefet; S Kaul-Strehlow; C J Lowe; P Flammang; E Ullrich-Lüter; A Wanninger; M I Arnone

doi:10.1016/j.margen.2015.10.001

Opsin evolution in the Ambulacraria

Mar Genomics. 2015 Dec:24 Pt 2:177-83. doi: 10.1016/j.margen.2015.10.001. Epub 2015 Oct 23.

Authors

S D'Aniello¹, J Delroisse², A Valero-Gracia³, E K Lowe⁴, M Byrne⁵, J T Cannon⁶, K M Halanych⁷, M R Elphick⁸, J Mallefet⁹, S Kaul-Strehlow¹⁰, C J Lowe¹¹, P Flammang¹², E Ullrich-Lüter¹³, A Wanninger¹⁴, M I Arnone³

Affiliations

¹ Department of Biology and Evolution of Marine Organisms, Stazione Zoologica Anton Dohrn, Villa Comunale, 80121 Napoli, Italy. Electronic address: salvatore.daniello@szn.it.
² Biology of Marine Organisms and Biomimetics, Research Institute for Biosciences, University of Mons, Avenue du Champs de Mars 6, 7000 Mons, Belgium; School of Biological & Chemical Sciences, Queen Mary University of London, London E1 4NS, UK.
³ Department of Biology and Evolution of Marine Organisms, Stazione Zoologica Anton Dohrn, Villa Comunale, 80121 Napoli, Italy.
⁴ Department of Biology and Evolution of Marine Organisms, Stazione Zoologica Anton Dohrn, Villa Comunale, 80121 Napoli, Italy; BEACON Center for the Study of Evolution in Action, Michigan State University, East Lansing, MI, USA.
⁵ Schools of Medical and Biological Sciences, The University of Sydney, Sydney, NSW, Australia.
⁶ Department of Biological Sciences and Molette Biology Laboratory for Environmental and Climate Change Studies, Auburn University, Auburn, USA; Department of Zoology, Naturhistoriska Riksmuseet, Stockholm, Sweden; Friday Harbor Laboratories, University of Washington, Friday Harbor, WA 98250, USA.
⁷ Department of Biological Sciences and Molette Biology Laboratory for Environmental and Climate Change Studies, Auburn University, Auburn, USA.
⁸ School of Biological & Chemical Sciences, Queen Mary University of London, London E1 4NS, UK.
⁹ Laboratory of Marine Biology, Earth and Life Institute, Université Catholique de Louvain, Louvain-La-Neuve, Place Croix du Sud 3, bt L7.06.04, 1348 Louvain-la-Neuve, Belgium.
¹⁰ Department of Molecular Evolution and Development, University of Vienna, Althanstrasse 14, 1090 Vienna, Austria.
¹¹ Hopkins Marine Station of Stanford University, Pacific Grove, CA 93950, USA.
¹² Biology of Marine Organisms and Biomimetics, Research Institute for Biosciences, University of Mons, Avenue du Champs de Mars 6, 7000 Mons, Belgium.
¹³ Museum fuer Naturkunde Berlin, Invalidenstr 43, 10115 Berlin, Germany.
¹⁴ Department of Integrative Zoology, University of Vienna, Althanstrasse 14, 1090 Vienna, Austria.

PMID: 26472700
DOI: 10.1016/j.margen.2015.10.001

Abstract

Opsins--G-protein coupled receptors involved in photoreception--have been extensively studied in the animal kingdom. The present work provides new insights into opsin-based photoreception and photoreceptor cell evolution with a first analysis of opsin sequence data for a major deuterostome clade, the Ambulacraria. Systematic data analysis, including for the first time hemichordate opsin sequences and an expanded echinoderm dataset, led to a robust opsin phylogeny for this cornerstone superphylum. Multiple genomic and transcriptomic resources were surveyed to cover each class of Hemichordata and Echinodermata. In total, 119 ambulacrarian opsin sequences were found, 22 new sequences in hemichordates and 97 in echinoderms (including 67 new sequences). We framed the ambulacrarian opsin repertoire within eumetazoan diversity by including selected reference opsins from non-ambulacrarians. Our findings corroborate the presence of all major ancestral bilaterian opsin groups in Ambulacraria. Furthermore, we identified two opsin groups specific to echinoderms. In conclusion, a molecular phylogenetic framework for investigating light-perception and photobiological behaviors in marine deuterostomes has been obtained.

Keywords: Ambulacraria; Echinoderm; Echinopsin; Hemichordate; Opsin; Photoreceptor cell evolution; Phylogeny.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Amino Acid Sequence
Animals
Chordata, Nonvertebrate / genetics*
Echinodermata / genetics*
Evolution, Molecular*
Gene Expression Regulation
Models, Molecular
Molecular Sequence Data
Opsins / genetics
Opsins / metabolism*
Phylogeny*
Protein Conformation

Substances

Opsins

Grants and funding

BB/M001644/1/Biotechnology and Biological Sciences Research Council/United Kingdom