Distribution and characterization of Clostridium difficile isolated from dogs in Japan

Masaru Usui; Kaname Suzuki; Kentaro Oka; Kentaro Miyamoto; Motomichi Takahashi; Takashi Inamatsu; Shigeru Kamiya; Yutaka Tamura

doi:10.1016/j.anaerobe.2015.10.002

Distribution and characterization of Clostridium difficile isolated from dogs in Japan

Anaerobe. 2016 Feb:37:58-61. doi: 10.1016/j.anaerobe.2015.10.002. Epub 2015 Oct 9.

Authors

Masaru Usui¹, Kaname Suzuki¹, Kentaro Oka², Kentaro Miyamoto², Motomichi Takahashi³, Takashi Inamatsu⁴, Shigeru Kamiya⁵, Yutaka Tamura⁶

Affiliations

¹ Laboratory of Food Microbiology and Food Safety, Department of Health and Environmental Sciences, School of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan.
² Miyarisan Pharmaceutical Co., Ltd., Tokyo, Japan.
³ Miyarisan Pharmaceutical Co., Ltd., Tokyo, Japan; Department of Infectious Diseases, Kyorin University School of Medicine, Tokyo, Japan.
⁴ Department of Infectious Diseases, Tokyo Metropolitan Geriatric Hospital, Tokyo, Japan.
⁵ Department of Infectious Diseases, Kyorin University School of Medicine, Tokyo, Japan.
⁶ Laboratory of Food Microbiology and Food Safety, Department of Health and Environmental Sciences, School of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan. Electronic address: tamuray@rakuno.ac.jp.

PMID: 26456188
DOI: 10.1016/j.anaerobe.2015.10.002

Abstract

We collected 204 nondiarrhoeic canine fecal samples and isolated 68 Clostridium difficile strains from 62 of these samples. Strains were grouped into 29 PCR ribotypes. Only 47% of the strains were toxigenic.

Keywords: Antimicrobial resistance; Clostridium difficile; Dogs; PCR ribotype; PFGE; Whole-genome sequencing.

MeSH terms

Animals
Clostridioides difficile / classification
Clostridioides difficile / isolation & purification*
Dogs / microbiology*
Feces / microbiology
Female
Japan
Male
Pilot Projects
Polymerase Chain Reaction / veterinary
Ribotyping / veterinary