A novel self-assembled nano micelle as a highly efficient artificial peroxidase based on hexadecyl trimethyl ammonium bromide and cytochrome c

Biomed Mater Eng. 2015:26 Suppl 1:S73-9. doi: 10.3233/BME-151291.

Abstract

A novel artificial peroxidase (AP) with highly catalytic efficiency was designed using hexadecyl trimethyl ammonium bromide (CTAB, 3 mM) nano-micelles and bovine heart cytochrome c (Cyt c, 0.5 µM) in 100 mM, pH 8.0 phosphate buffer at 25°C. The catalytic rate (kcat) and Michaelis-Menten (Km) of the AP were determined to be 0.311 ± 0.013 s(-1) and 8.64 ± 0.6 µM. The catalytic efficiency was 0.0360 ± 0.0020 µM(-1)s(-1) (about 50% the efficiency of native horseradish peroxidase). The Ultraviolet-visible spectrophotometer and Circular Dichroism techniques were applied to study the properties of the CTAB-Cyt c nano-micelle. Designed AP can be applied instead of native horseradish peroxidase.

Keywords: CTAB; Catalytic efficiency; artificial peroxidase; cytochrome c; nano-micelle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomimetic Materials / chemical synthesis*
  • Catalysis
  • Cetrimonium
  • Cetrimonium Compounds / chemistry*
  • Cytochromes c / chemical synthesis*
  • Enzyme Activation
  • Enzyme Stability
  • Materials Testing
  • Micelles
  • Nanocomposites / chemistry*
  • Nanocomposites / ultrastructure*
  • Particle Size
  • Peroxidase / chemical synthesis*
  • Substrate Specificity

Substances

  • Cetrimonium Compounds
  • Micelles
  • Cytochromes c
  • Peroxidase
  • Cetrimonium