Cloning of a thermostable alpha-amylase gene from Thermomonospora curvata and its expression in Streptomyces lividans

J Gen Microbiol. 1989 Dec;135(12):3303-9. doi: 10.1099/00221287-135-12-3303.

Abstract

The gene from Thermomonospora curvata CCM 3312 coding for thermostable alpha-amylase (tam) has been cloned in Streptomyces lividans TK 24 and localized to a 2.6 kb HindIII-BamHI fragment of DNA. The data presented here show that the tam gene is expressed at a high level in S. lividans and that the protein is efficiently excreted.

MeSH terms

  • Actinomycetaceae / enzymology*
  • Cloning, Molecular
  • DNA, Bacterial
  • Electrophoresis, Polyacrylamide Gel
  • Gene Expression Regulation, Bacterial*
  • Genes, Bacterial
  • Genes, Fungal
  • Hot Temperature
  • Restriction Mapping
  • Streptomyces / enzymology
  • Streptomyces / genetics*
  • alpha-Amylases / biosynthesis
  • alpha-Amylases / genetics*

Substances

  • DNA, Bacterial
  • alpha-Amylases