Genomic Assays for Identification of Chikungunya Virus in Blood Donors, Puerto Rico, 2014

Emerg Infect Dis. 2015 Aug;21(8):1409-13. doi: 10.3201/eid2108.150458.

Abstract

A newly developed transcription-mediated amplification assay was used to detect chikungunya virus infection in 3 of 557 asymptomatic donors (0.54%) from Puerto Rico during the 2014-2015 Caribbean epidemic. Viral detection was confirmed by using PCR, microarray, and next-generation sequencing. Molecular clock analysis dated the emergence of the Puerto Rico strains to early 2013.

Keywords: ViroChip microarray; blood donor; chikungunya virus; next-generation sequencing; transcription-mediated amplification; transfusion-transmitted infection; viruses; whole-genome sequencing.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blood Donors / statistics & numerical data
  • Blood Donors / supply & distribution*
  • Chikungunya Fever / diagnosis*
  • Chikungunya Fever / epidemiology
  • Chikungunya Fever / genetics
  • Chikungunya virus / genetics
  • Chikungunya virus / immunology
  • Chikungunya virus / isolation & purification*
  • Chikungunya virus / pathogenicity
  • Genetic Testing / methods*
  • Genetic Testing / statistics & numerical data
  • Genomics*
  • Humans
  • Puerto Rico / epidemiology