A highly selective fluorescent probe for direct detection and isolation of mouse embryonic stem cells

Bioorg Med Chem Lett. 2015 Nov 1;25(21):4862-4865. doi: 10.1016/j.bmcl.2015.06.037. Epub 2015 Jun 16.

Abstract

Stem cell research has gathered immense attention in the past decade due to the remarkable ability of stem cells for self-renewal and tissue-specific differentiation. Despite having numerous advancements in stem cell isolation and manipulation techniques, there is a need for highly reliable probes for the specific detection of live stem cells. Herein we developed a new fluorescence probe (CDy9) with high selectivity for mouse embryonic stem cells. CDy9 allows the detection and isolation of intact stem cells with marginal impact on their function and capabilities.

Keywords: Fluorescence imaging; Fluorescence-assisted cell sorting; Mouse embryonic stem cells; Single cell PCR; Three germ layers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Boron Compounds / analysis
  • Boron Compounds / chemistry*
  • Cell Separation / methods*
  • Fluorescent Dyes / analysis
  • Fluorescent Dyes / chemistry*
  • Heterocyclic Compounds, 3-Ring / analysis
  • Heterocyclic Compounds, 3-Ring / chemistry*
  • Mice
  • Molecular Structure
  • Mouse Embryonic Stem Cells / cytology*

Substances

  • Boron Compounds
  • CDy9 compound
  • Fluorescent Dyes
  • Heterocyclic Compounds, 3-Ring