Solution Structure of CCL19 and Identification of Overlapping CCR7 and PSGL-1 Binding Sites

Biochemistry. 2015 Jul 14;54(27):4163-6. doi: 10.1021/acs.biochem.5b00560. Epub 2015 Jul 6.

Abstract

CCL19 and CCL21 are chemokines involved in the trafficking of immune cells, particularly within the lymphatic system, through activation of CCR7. Concurrent expression of PSGL-1 and CCR7 in naive T-cells enhances recruitment of these cells to secondary lymphoid organs by CCL19 and CCL21. Here the solution structure of CCL19 is reported. It contains a canonical chemokine domain. Chemical shift mapping shows the N-termini of PSGL-1 and CCR7 have overlapping binding sites for CCL19 and binding is competitive. Implications for the mechanism of PSGL-1's enhancement of resting T-cell recruitment are discussed.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Binding Sites
  • Chemokine CCL19 / chemistry*
  • Chemokine CCL19 / metabolism*
  • Humans
  • Membrane Glycoproteins / metabolism*
  • Models, Molecular
  • Protein Conformation
  • Receptors, CCR7 / metabolism*

Substances

  • CCR7 protein, human
  • Chemokine CCL19
  • Membrane Glycoproteins
  • P-selectin ligand protein
  • Receptors, CCR7

Associated data

  • PDB/2MP1