Simultaneous determination of trimethoprim and sulfamethoxazole in dried plasma and urine spots

Daniel Gonzalez; Chiara Melloni; Brenda B Poindexter; Ram Yogev; Andrew M Atz; Janice E Sullivan; Susan R Mendley; Paula Delmore; Amy Delinsky; Kanecia Zimmerman; Andrew Lewandowski; Barrie Harper; Kenneth C Lewis; Daniel K Benjamin Jr; Michael Cohen-Wolkowiez; Best Pharmaceuticals for Children Act--Pediatric Trials Network Administrative Core Committee

doi:10.4155/bio.15.38

Simultaneous determination of trimethoprim and sulfamethoxazole in dried plasma and urine spots

Bioanalysis. 2015;7(9):1137-49. doi: 10.4155/bio.15.38.

Authors

Daniel Gonzalez, Chiara Melloni¹, Brenda B Poindexter², Ram Yogev, Andrew M Atz³, Janice E Sullivan⁴, Susan R Mendley⁵, Paula Delmore⁶, Amy Delinsky⁷, Kanecia Zimmerman¹, Andrew Lewandowski⁸, Barrie Harper¹, Kenneth C Lewis⁷, Daniel K Benjamin Jr^{1

9}, Michael Cohen-Wolkowiez^{1

9}; Best Pharmaceuticals for Children Act--Pediatric Trials Network Administrative Core Committee

Collaborators

Best Pharmaceuticals for Children Act--Pediatric Trials Network Administrative Core Committee:
K Y Berezny, E Capparelli, G L Kearns, M Laughon, A Muelenaer, T M O'Shea, I M Paul, P B Smith, J van den Anker, K Wade, T J Walsh, D Siegel, P Taylor-Zapata, A Zajicek, Z Ren, K Tsilou, A Pagan, R Anand, G Simone, A Lewandowski, B Harper, B Poindexter, L Smiley, R Yogev, L Fern, Andrew Atz, H Al Nasiri, J Sullivan, T Bratton, Susan Mendley, Donna Cannonier, Paula Delmore

Affiliations

¹ 2Duke Clinical Research Institute, Duke University Medical Center, Durham, NC, USA.
² 3Department of Pediatrics, Riley Hospital for Children, Indiana University, Indianapolis, IN, USA.
³ 5Department of Pediatrics, Medical University of South Carolina, Charleston, SC, USA.
⁴ 6Kosair Charities Pediatric Clinical Research Unit, Department of Pediatrics, University of Louisville, Kosair Children's Hospital, Louisville, KY, USA.
⁵ 7Department of Pediatrics, University of Maryland School of Medicine, Baltimore, MD, USA.
⁶ 8Department of Neonatology, Wesley Medical Center, Wichita, KS, USA.
⁷ 9OpAns, LLC, 4134 S Alston Ave # 101, Durham, NC 27713, USA.
⁸ 10EMMES Corp., 401 N Washington St # 700, Rockville, MD 20850, USA.
⁹ 11Department of Pediatrics, Duke University Medical Center, Durham, NC, USA.

Abstract

Background: Trimethoprim-sulfamethoxazole (TMP-SMX) is an antimicrobial drug combination commonly prescribed in children and adults. The study objectives were to validate and apply an HPLC-MS/MS method to quantify TMP-SMX in dried plasma spots (DPS) and dried urine spots (DUS), and perform a comparability analysis with liquid matrices.

Results: For TMP the validated range was 100-50,000 ng/ml for DPS and 500-250,000 ng/ml for DUS; for SMX, the validated range was 1000-500,000 ng/ml for both DPS and DUS. Good agreement was noted between DPS/DUS and liquid plasma and urine samples for TMP, while only modest agreement was observed for SMX in both matrices.

Conclusion: A precise, accurate and reproducible method was developed to quantify TMP-SMX in DPS and DUS samples.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Adult
Calibration
Child
Chromatography, High Pressure Liquid
Dried Blood Spot Testing / methods*
Humans
Tandem Mass Spectrometry
Time Factors
Trimethoprim, Sulfamethoxazole Drug Combination / blood*
Trimethoprim, Sulfamethoxazole Drug Combination / urine*
Urinalysis / methods*

Substances

Trimethoprim, Sulfamethoxazole Drug Combination

Abstract

Publication types

MeSH terms

Substances

Grants and funding