Increase of high molecular weight adiponectin in hypertensive pregnancy was correlated with brain-type natriuretic peptide stimulation on adipocyte

Katsuhiko Naruse; Yui Yamasaki; Taihei Tsunemi; Akira Onogi; Taketoshi Noguchi; Toshiyuki Sado; Hidekazu Oi; Hiroshi Kobayashi

doi:10.1016/j.preghy.2011.07.004

Increase of high molecular weight adiponectin in hypertensive pregnancy was correlated with brain-type natriuretic peptide stimulation on adipocyte

Pregnancy Hypertens. 2011 Jul-Oct;1(3-4):200-5. doi: 10.1016/j.preghy.2011.07.004. Epub 2011 Jul 26.

Authors

Katsuhiko Naruse¹, Yui Yamasaki², Taihei Tsunemi³, Akira Onogi³, Taketoshi Noguchi³, Toshiyuki Sado³, Hidekazu Oi³, Hiroshi Kobayashi³

Affiliations

¹ Department of Obstetrics and Gynecology, Nara Medical University, Nara, Japan. Electronic address: naruse@naramed-u.ac.jp.
² Center for Postgraduate Training, Nara Medical University, Nara, Japan.
³ Department of Obstetrics and Gynecology, Nara Medical University, Nara, Japan.

PMID: 26009027
DOI: 10.1016/j.preghy.2011.07.004

Abstract

Background: High-molecular weight (HMW)-adiponectin is an active multimer for insulin sensitivity and anti-inflammatory reactions. We compared the ratio of serum total and HMW-adiponectin with brain-type natriuretic peptide (BNP) and other adipocytokines in normal pregnancy and pregnancy-induced hypertension (PIH). Effect of BNP on the secretion of adiponectin from cultured adipocytes was also examined.

Methods: The three study groups consisted of 44 non-pregnant women, 40 normal (healthy) pregnant women over 28weeks gestation and 29 patients with severe PIH. Adiponectin (protease-pretreated for HMW), BNP-N-terminal, leptin, and monocyte chemoattractant protein (MCP)-1 were measured with ELISA. Pre-adipocytes were differentiated to matured adipocytes and cultured with recombinant-BNP addition.

Results: HMW-to-total adiponectin ratio (HMW-ratio) was lower in normal pregnancy than in non-pregnant, and significantly higher in PIH than normal pregnancies. BNP-N-terminal showed positive correlation with HMW-adiponectin and HMW-ratio. Leptin and MCP-1 showed positive correlation with HMW-adiponectin, but not with HMW-ratio. Adiponectin in the supernatant of adipocyte cultures and intracellular cyclic-GMP was increased in dose-dependent manner in response to BNP.

Conclusion: The observed increase in the HMW-adponectin ratio in subjects with PIH may reflect a functional increase of adiponectin in the pathophysiology of PIH. Additionally, this increase seemed to be related to BNP via stimulation of adipocytes.

Keywords: Adipocytokine; Adiponectin; Brain-type natriuretic peptide; Insulin resistance; Pregnancy induced hypertension.