Identification of a 6-cytokine prognostic signature in patients with primary glioblastoma harboring M2 microglia/macrophage phenotype relevance

PLoS One. 2015 May 15;10(5):e0126022. doi: 10.1371/journal.pone.0126022. eCollection 2015.

Abstract

Background: Glioblastomas (GBM) are comprised of a heterogeneous population of tumor cells, immune cells, and extracellular matrix. Interactions among these different cell types and pro-/anti-inflammatory cytokines may promote tumor development and progression.

Aims: The objective of this study was to develop a cytokine-related gene signature to improve outcome prediction for patients with primary GBM.

Methods: Here, we used Cox regression and risk-score analysis to develop a cytokine-related gene signature in primary GBMs from the whole transcriptome sequencing profile of the Chinese Glioma Genome Atlas (CGGA) database (n=105). We also examined differences in immune cell phenotype and immune factor expression between the high-risk and low-risk groups.

Results: Cytokine-related genes were ranked based on their ability to predict survival in the CGGA database. The six genes showing the strongest predictive value were CXCL10, IL17R, CCR2, IL17B, IL10RB, and CCL2. Patients with a high-risk score had poor overall survival and progression-free survival. Additionally, the high-risk group was characterized by increased mRNA expression of M2 microglia/macrophage markers and elevated levels of IL10 and TGFβ1.

Conclusion: The six cytokine-related gene signature is sufficient to predict survival and to identify a subgroup of primary GBM exhibiting the M2 cell phenotype.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Brain Neoplasms / diagnosis*
  • Brain Neoplasms / genetics
  • Brain Neoplasms / mortality
  • Brain Neoplasms / pathology
  • Chemokine CCL2 / genetics
  • Chemokine CXCL10 / genetics
  • Cytokines / genetics*
  • DNA Methylation
  • Glioblastoma / diagnosis*
  • Glioblastoma / genetics
  • Glioblastoma / mortality
  • Glioblastoma / pathology
  • Humans
  • Interleukin-10 Receptor beta Subunit / genetics
  • Interleukin-17 / genetics
  • Macrophages / pathology*
  • Microglia / pathology*
  • Middle Aged
  • Phenotype
  • Prognosis
  • Proportional Hazards Models
  • Receptors, CCR2 / genetics
  • Receptors, Interleukin-17 / genetics
  • Risk Factors
  • Survival Analysis
  • Transcriptome
  • Young Adult

Substances

  • CCL2 protein, human
  • CCR2 protein, human
  • CXCL10 protein, human
  • Chemokine CCL2
  • Chemokine CXCL10
  • Cytokines
  • IL10RB protein, human
  • IL17B protein, human
  • IL17RA protein, human
  • Interleukin-10 Receptor beta Subunit
  • Interleukin-17
  • Receptors, CCR2
  • Receptors, Interleukin-17

Grants and funding

This work was supported by the following sources of funding: 1. National High Technology Research and Development Program (863) (No. 2012AA02A508); 2. International Science and Technology Cooperation Program (No. 2012DFA30470); 3. National Natural Science Foundation of China (No. 91229121, No. 81201993); 4. Project of Ministry of Health P. R. China (No. w2011bx059); 5. National 973 program (No. 2011CB707804).