Accuracy of in vitro susceptibility tests for carbapenemase-producing Gram-negative bacteria

J Med Microbiol. 2015 Jun;64(6):620-622. doi: 10.1099/jmm.0.000067. Epub 2015 Apr 14.

Abstract

Accurate susceptibility results on antibiotic-resistant bacteria are essential for proper treatment of infections. In this study, 100 metallo-β-lactamase (MBL)-producing strains and 95 isolates with Klebsiella pneumoniae carbapenemase (KPC) were tested for carbapenem susceptibility using two automated platforms, the Phoenix and Vitek-2 systems, and a manual Etest. Phoenix showed higher categorical agreements (97% for imipenem and 94% for meropenem) compared with those from Vitek-2 (92 and 74%) and Etest (89 and 96%), respectively, when testing MBL strains. Categorical agreement for imipenem tests with KPC-producing strains was 88.4% with the Phoenix system, 83.2% with the Vitek 2 system and 90.5% with the Etest. Categorical agreement was 100% for all tests with ertapenem. In conclusion, the Phoenix system demonstrated a higher accuracy than Vitek-2 in testing carbapenemase-producing strains, particularly in MBL strains.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Automation, Laboratory / methods
  • Bacterial Proteins / metabolism*
  • Diagnostic Errors
  • Gram-Negative Bacteria / drug effects*
  • Gram-Negative Bacteria / enzymology*
  • Humans
  • Microbial Sensitivity Tests / methods*
  • beta-Lactamases / metabolism*
  • beta-Lactams / pharmacology*

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • beta-Lactams
  • beta-Lactamases
  • carbapenemase