A novel platform based on immobilized histidine tagged olfactory receptors, for the amperometric detection of an odorant molecule characteristic of boar taint

ZhenZhong Guo; Nadia Zine; Florence Lagarde; Julien Daligault; Marie-Annick Persuy; Edith Pajot-Augy; Aidong Zhang; Nicole Jaffrezic-Renault

doi:10.1016/j.foodchem.2015.03.066

A novel platform based on immobilized histidine tagged olfactory receptors, for the amperometric detection of an odorant molecule characteristic of boar taint

Food Chem. 2015 Oct 1:184:1-6. doi: 10.1016/j.foodchem.2015.03.066. Epub 2015 Mar 26.

Authors

ZhenZhong Guo¹, Nadia Zine¹, Florence Lagarde¹, Julien Daligault², Marie-Annick Persuy², Edith Pajot-Augy², Aidong Zhang³, Nicole Jaffrezic-Renault⁴

Affiliations

¹ University of Lyon, Institute of Analytical Chemistry, LSA Department, 5 Rue de la Doua, 69100 Villeurbanne, France.
² INRA, UR1197 Neurobiologie de l'Olfaction et Modélisation en Imagerie, F-78350 Jouy-en-Josas, France; IFR 144, NeuroSud Paris, F-91190 Gif-Sur-Yvette, France.
³ Key Laboratory of Pesticides and Chemical Biology, Huazhong Normal University, Luoyu Str 152, Hongshan District, 430079 Wuhan, China.
⁴ University of Lyon, Institute of Analytical Chemistry, LSA Department, 5 Rue de la Doua, 69100 Villeurbanne, France. Electronic address: nicole.jaffrezic@univ-lyon1.fr.

PMID: 25872419
DOI: 10.1016/j.foodchem.2015.03.066

Abstract

We report a dose-dependent detection of androstenone in solution, as one of the boar taint compounds, based on related OR7D4 olfactory receptors immobilized on a gold electrode through their 6-His tag and NTA-copper complex, as visualized through fluorescence microscopy. Square wave voltammetry (SWV) is for the first time, the method used to monitor the olfactory receptor/odorant recognition process. The relative variation of the Cu(I)-Cu(II) current peak increases linearly versus log (concentration of androstenone) from 10(-14)M to 10(-4)M, in buffer solution. Negative tests were performed, using an unrelated odorant, helional, itself a ligand of OR 1740. Cross-selectivity was also tested after immobilization of OR 1740.

Keywords: Fluorescence microscopy; Functional immobilization; Olfactory receptor; Square wave voltammetry.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Androstenes / analysis*
Animals
Electrochemical Techniques*
Gold
Histidine / chemistry*
Immobilized Proteins / chemistry*
Male
Microscopy, Fluorescence
Receptors, Odorant / chemistry*
Swine

Substances

Androstenes
Immobilized Proteins
Receptors, Odorant
androst-16-en-3-one
Histidine
Gold