Effect of acute NR2B antagonist treatment on long-term potentiation in the rat hippocampus

Brain Res. 2015 Jun 3:1609:31-9. doi: 10.1016/j.brainres.2015.03.019. Epub 2015 Mar 19.

Abstract

The long lasting antidepressant response seen following acute, i.v. ketamine administration in patients with treatment-resistant depression (TRD) is thought to result from enhanced synaptic plasticity in cortical and hippocampal circuits. Using extracellular field recordings in rat hippocampal slices, we show that a single dose of the non-selective NMDA receptor antagonist ketamine or CP-101,606, a selective antagonist of the NR2B subunit of the NMDA receptor, enhances hippocampal synaptic plasticity induced with high frequency stimulation (HFS) 24h after dosing - a time at which plasma concentrations of the drug are no longer detectable in the animal. These results indicate that acute inhibition of NMDA receptors containing the NR2B subunit can lead to long-lasting changes in hippocampal plasticity.

Keywords: Ex vivo; Hippocampus; Long-term potentiation; NMDA receptors; NR2B subunit; Plasticity; Treatment-resistant depression.

MeSH terms

  • Animals
  • Antidepressive Agents / pharmacokinetics
  • Antidepressive Agents / pharmacology
  • Electric Stimulation / methods
  • Excitatory Amino Acid Antagonists / pharmacokinetics
  • Excitatory Amino Acid Antagonists / pharmacology*
  • Hippocampus / drug effects*
  • Hippocampus / physiology
  • Ketamine / pharmacology*
  • Long-Term Potentiation / drug effects*
  • Long-Term Potentiation / physiology
  • Male
  • Piperidines / pharmacokinetics
  • Piperidines / pharmacology*
  • Rats, Sprague-Dawley
  • Receptors, N-Methyl-D-Aspartate / antagonists & inhibitors*
  • Receptors, N-Methyl-D-Aspartate / metabolism
  • Time Factors
  • Tissue Culture Techniques

Substances

  • Antidepressive Agents
  • Excitatory Amino Acid Antagonists
  • NR2B NMDA receptor
  • Piperidines
  • Receptors, N-Methyl-D-Aspartate
  • Ketamine
  • traxoprodil mesylate