Determining antioxidant activities of lactobacilli cell-free supernatants by cellular antioxidant assay: a comparison with traditional methods

Jiali Xing; Gang Wang; Qiuxiang Zhang; Xiaoming Liu; Zhennan Gu; Hao Zhang; Yong Q Chen; Wei Chen

doi:10.1371/journal.pone.0119058

Determining antioxidant activities of lactobacilli cell-free supernatants by cellular antioxidant assay: a comparison with traditional methods

PLoS One. 2015 Mar 19;10(3):e0119058. doi: 10.1371/journal.pone.0119058. eCollection 2015.

Authors

Jiali Xing¹, Gang Wang¹, Qiuxiang Zhang¹, Xiaoming Liu¹, Zhennan Gu¹, Hao Zhang¹, Yong Q Chen¹, Wei Chen²

Affiliations

¹ State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, China.
² State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, China; Synergistic Innovation Center for Food Safety and Nutrition, Wuxi, China.

Abstract

Antioxidant activity of lactic acid bacteria is associated with multiple health-protective effects. Traditional indexes of chemical antioxidant activities poorly reflect the antioxidant effects of these bacteria in vivo. Cellular antioxidant activity (CAA) assay was used in this study to determine the antioxidant activity of cell-free supernatants (CFSs) of 10 Lactobacillus strains. The performance of the CAA assay was compared with that of four chemical antioxidant activity assays, namely, DPPH radical scavenging, hydroxyl radical scavenging (HRS), reducing power (RP), and inhibition of linoleic acid peroxidation (ILAP). Results of the CAA assay were associated with those of DPPH and ILAP assays, but not with those of RP and HRS assays. The inter- and intra-specific antioxidant activities of CFS were characterized by chemical and CAA assays. L. rhamnosus CCFM 1107 displayed a high antioxidative effect similar to positive control L. rhamnosus GG ATCC 53103 in all of the assays. The CAA assay is a potential method for the detection of antioxidant activities of lactobacilli CFSs.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Antioxidants / metabolism*
Biological Assay / methods*
Biphenyl Compounds / chemistry
Cell-Free System
Free Radical Scavengers / chemistry
Hep G2 Cells
Humans
Hydroxyl Radical / chemistry
Lactobacillus / metabolism*
Linoleic Acid / chemistry
Lipid Peroxidation
Oxidation-Reduction
Picrates / chemistry

Substances

Antioxidants
Biphenyl Compounds
Free Radical Scavengers
Picrates
Hydroxyl Radical
Linoleic Acid
1,1-diphenyl-2-picrylhydrazyl

Grants and funding

This work was supported by the National High Technology Research and Development Program of China (863 Program Nos. 2013BAD18B01, 2013BAD18B02, 2012BAD28B07, 2012BAD12B08), the National Natural Science Foundation of China (Nos. 31301407, 31200691), the National Basic Research Program of China (973 Program No. 2012CB720802), key projects in the National Science and Technology Pillar Program during the 12th Five- Year Plan (Nos. 2012BAD12B08, 2012BAD28B07), the 111 project B07029, and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.