Expression of Bcl-xL and Mcl-1 in the nonmelanoma skin cancers of renal transplant recipients

Am J Clin Pathol. 2015 Apr;143(4):514-26. doi: 10.1309/AJCPQNB5WA3PLQBK.

Abstract

Objectives: This study aims to investigate how immunosuppression influences the protein expression of antiapoptotic members of the Bcl-2 family-namely, Bcl-xL and Mcl-1-in nonmelanoma skin cancer (NMSC) and the peritumoral epidermis of renal transplant recipients.

Methods: NMSC and peritumoral epidermis protein expression of Bcl-xL and Mcl-1 were assessed by immunohistochemistry in renal transplant recipients receiving tacrolimus or sirolimus and the general population not receiving immunosuppression.

Results: NMSC from renal transplant recipients compared with patients not receiving immunosuppressant medications had a reduced Bcl-xL expression intensity (P = .042). Mcl-1 expression intensity in NMSC was decreased in tacrolimus-treated patients compared with sirolimus-treated patients and the nonimmunosuppressed population (P = .024). Bcl-xL expression intensity was increased in peritumoral epidermis compared with NMSC (P = .002).

Conclusions: It was shown for the first time that Bcl-xL and Mcl-1 expression are widespread in the peritumoral epidermis and NMSC of renal transplant recipients. Importantly in NMSC, Bcl-xL expression was reduced with immunosuppression exposure, and Mcl-1 expression was reduced in tacrolimus-treated compared with sirolimus-treated patients.

Keywords: Apoptosis; Bcl-xL; Immunohistochemistry; Mcl-1; Mitosis; Nonmelanoma skin cancer; Renal transplantation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Apoptosis
  • Carcinoma, Basal Cell / metabolism*
  • Carcinoma, Basal Cell / pathology
  • Carcinoma, Squamous Cell / metabolism*
  • Carcinoma, Squamous Cell / pathology
  • Female
  • Humans
  • Immunohistochemistry
  • Immunosuppressive Agents / therapeutic use*
  • Kidney Transplantation
  • Male
  • Middle Aged
  • Mitosis
  • Myeloid Cell Leukemia Sequence 1 Protein / metabolism*
  • Sirolimus / therapeutic use
  • Skin Neoplasms / metabolism*
  • Skin Neoplasms / pathology
  • Tacrolimus / therapeutic use
  • Transplant Recipients
  • bcl-X Protein / metabolism*

Substances

  • BCL2L1 protein, human
  • Immunosuppressive Agents
  • MCL1 protein, human
  • Myeloid Cell Leukemia Sequence 1 Protein
  • bcl-X Protein
  • Sirolimus
  • Tacrolimus